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pAC8-HUWE1-HECTonly

Plasmid

#187119

Purpose

insect cell expression of HUWE1 HECT domain

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

Strep

Expression

Insect

Mutation

delta1-3992

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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puc19_N_tag

Plasmid

#186650

Purpose

Empty donor plasmid.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

N-terminal 3xFlag-3xHA Tag

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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attB53-Pac-tetO-mCherry-attB53

Plasmid

#183612

Purpose

RMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

tetO-mCherry

Use

Rmce

Promoter

tetO

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pB80-SSPB(micro)-mVenus-ppKin14VIb(861-1321)

Plasmid

#174641

Purpose

Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

SSPB(micro)-mVenus-ppKin14VIb(861-1321)

Tags

SSPB(micro)-Venus

Expression

Mammalian

Mutation

SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

Promoter

Chicken beta-actin

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 MYC dBR

Plasmid

#176046

Purpose

Expresses Myc Mutant (delta basic region) in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Expression

Mammalian

Mutation

deleted amino acids 355-369

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-loxP-canisNrg1

Plasmid

#173849

Purpose

Encoding dog Nrg1 before Cre-mediated recombination.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis Nrg1

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-canisEgf

Plasmid

#173845

Purpose

Encoding dog Egf.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A cDNA of canis EGF.

Expression

Mammalian

Mutation

R1127Q- please see dep. comments

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-squirrel-monkey-CHMP3(155)

Plasmid

#154183

Purpose

expresses squirrel monkey CHMP3(155) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3(155)

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 155

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCK-D390(UniProt)-FLAG

Plasmid

#164692

Purpose

Express N-terminally truncated Drosha-FLAG in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

N-terminally truncated Drosha (DROSHA Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144[309 nt]~451-CMV2-mir1

Plasmid

#164680

Purpose

Express miR-144[309 nt]~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144[309 nt]~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir451~451-CMV2-mir1

Plasmid

#164667

Purpose

Express miR-451~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-451~451 (MIR451A Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144m5~451-CMV2-mir1

Plasmid

#164679

Purpose

Express miR-144(mutant5)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant5)~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144m4~451-CMV2-mir1

Plasmid

#164678

Purpose

Express miR-144(mutant4)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant4)~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144m3~451-CMV2-mir1

Plasmid

#164677

Purpose

Express miR-144(mutant3)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant3)~451 (MIR144 Human)
mir-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144m2~451-CMV2-mir1

Plasmid

#164676

Purpose

Express miR-144(mutant2)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant2)~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144m1~451-CMV2-mir1

Plasmid

#164675

Purpose

Express miR-144(mutant1)~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144(mutant1)~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir125~451-CMV2-mir1

Plasmid

#164669

Purpose

Express miR-125a~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-125a~451 (MIR125A Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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Clim-DC2-H2B2-Venus

Plasmid

#129556

Purpose

pClimDC-H2B:mV nuclear marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

Histone 2B fused to venus

Expression

Bacterial

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Clim-DC2-lifeact-mCherry

Plasmid

#129558

Purpose

pClimDCLifeAct:mCh actin filaments marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

lifeAct fused to mCherry

Expression

Bacterial

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC2-GPT1-3xFLAG

Plasmid

#163446

Purpose

lentiviral overexpression vector for human GPT1

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

GPT1 (GPT Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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