165,373 results

1. pRK5_mCherry_P2AT2A_GFP-nb-2xKS

   Plasmid

   #238236

   Purpose

   For overexpression of mCherry_P2AT2A_GFP-nb-2xKS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb-2xKS
   

   Expression

   Mammalian

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWH1266-Apra-sgRNA

   Plasmid

   #218372

   Purpose

   sgRNA expression plasmid for CRISPR-intereference

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Insert

   sgRNA expression cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFlp-Km

   Plasmid

   #222334

   Purpose

   Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   sacB (sacB B. subtilis)
   flp (flp Budding Yeast)
   cI (cI )
   

   Promoter

   cl, flp, and sacB

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Peredox-mCherry

   Plasmid

   #32383

   Depositor

   Gary Yellen

   Article

   Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

   Insert

   Peredox-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. CDH5-AbR-GFP

   Plasmid

   #122970

   Purpose

   Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.

   Depositor

   Budd A. Tucker

   Inserts

   CDH5p
   CopGFP
   Zeocin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. slc1a3b:myrGFP-P2A-H2AmCherry

   Plasmid

   #170208

   Purpose

   Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

   Depositor

   Kelly Monk

   Article

   Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

   Insert

   slc1a3b (slc1a3b Zebrafish)
   

   Use

   Zebrafish

   Tags

   myrGFP-P2A-H2AmCherry

   Promoter

   slc1a3b

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pR26 CAG AsiSI/MluI

   Plasmid

   #74286

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX552

   Plasmid

   #60958

   Purpose

   pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

   Depositor

   Feng Zhang

   Article

   Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

   Inserts

   U6_(SpaI)_sgRNA
   Syn_EGFP-KASH
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Expression

   Mammalian

   Promoter

   Synapsin and U6

   Available Since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET_RTX

   Plasmid

   #102787

   Purpose

   Expresses the Reverse Transcriptase gene

   Depositor

   Andrew Ellington

   Article

   Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

   Insert

   reverse transcription xenopolymerase
   

   Expression

   Bacterial

   Promoter

   T7 promoter + lac operator

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. prDNA spacer-LacOx120-20xMS2-Hyg

    Plasmid

    #210220

    Purpose

    Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    120xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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