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pcDNA3.1_SM-FLuc100-malat1

Plasmid

#210581

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helix

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-EGFP

Plasmid

#73461

Purpose

Expresses EGFP for monosynaptic circuit mapping

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

EGFP

Use

Neurotropic virus

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_class_I_1

Plasmid

#164986

Purpose

Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA class I

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHCKan-phoAp-GFPuv

Plasmid

#139072

Purpose

Low phosphate inducible promoter phoAp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

phoAp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TYMS

Plasmid

#183326

Purpose

All-in-One CRISPRko system with a guide RNA that targets TYMS gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TYMS

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSUM36-MCS2-myc

Plasmid

#109379

Purpose

Shuttle vector for high level expression in mycobacteria.

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

MCS2-Myc

Tags

Myc

Expression

Bacterial

Promoter

Kanamycin

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_CdTag-HA

Plasmid

#221493

Purpose

NKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusion

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBT137-splitC

Plasmid

#122605

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

Plasmid

#64512

Purpose

Expression of St1 dCas9-3XTagBFP2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XmTagBFP2

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBT137-splitD

Plasmid

#122606

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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HLA_DRB1_04_01

Plasmid

#165013

Purpose

Expression of HLA-DRB1*04:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRB1*04:01 (HLA-DRB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTrc-GWS1B

Plasmid

#239582

Purpose

High copy vector for expression of GWS1B Rubisco

Depositor

Matthew D. Shoulders

Article

McDonald et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

GWS1B Rubisco (6X his)

Tags

6X histag

Expression

Bacterial

Mutation

Wild-type

Promoter

Trc

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_20bpLinker

Plasmid

#157787

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_20bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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ZJOM90

Plasmid

#133647

Purpose

Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

pTPI1-SS-mCherry-HDEL

Tags

mCherry

Expression

Yeast

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCMU-ACTFr

Plasmid

#61191

Purpose

Fluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtFimbrin1-ABD

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pNMT

Plasmid

#42578

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

N-myristoyl tranferase (NMT1 Budding Yeast)

Expression

Bacterial

Mutation

two silent mutations T690C and T942C

Promoter

T7

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only
PB-CAG-GFPd2

Plasmid

#115665

Purpose

Piggybac transposon plasmid for destabilized eGFP (GFPd2)

Depositor

Jordan Green

Article

Rui et al ACS Appl Mater Interfaces. 2019 Feb 22.

Insert

GFPd2

Use

Synthetic Biology

Tags

Destabilization

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-F98

Plasmid

#130704

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-F98

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMU-NUCr

Plasmid

#61168

Purpose

Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

NLS-mCherry-GUS

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCMU-ACTLr

Plasmid

#61193

Purpose

Fluorescent reporter for F-actin LifeAct-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LifeAct-mCherry

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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