We narrowed to 128,399 results for: CIL
-
Plasmid#221004PurposeTransiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCasRx 30nt processed direct repeat with SD1 mutation
UseCRISPRExpressionMammalianMutationDR1 A>TPromoterU6Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HA-Flag-natT1R2
Plasmid#113944Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutininDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-839Available sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-BRD4-7A
Plasmid#90006Purposeexpresses 7A mutant BRD4DepositorInsertBRD4 (BRD4 Human)
TagsFlagExpressionMammalianMutationChanged 484S to 484A, 488S to 488A, 492S to 492A,…Available sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKH3-TRIM28-C65A/C68A
Plasmid#92199PurposeMammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTif1b (TRIM28 Synthetic, Human)
TagsHAExpressionMammalianMutationChanged Cysteines 65 and 68 to Alanines.Available sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTol2pA2-ubb:secHsa.ANNEXINV-mVenus, cryaa:RFP
Plasmid#92388PurposeCell death reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertANNEXINV (ANXA5 Human)
UseZebrafish expressionTagsmVenusPromoterubbAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CTGF-bio-His
Plasmid#52028PurposeExpresses full-length Connective tissue growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCTGF (CCN2 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDest eGFP CUGBP1
Plasmid#61276PurposeMammalian expression of EGFP-tagged human CUGBP1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCUG-BP (CELF1 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBa.Kif5c 1-559.GFP
Plasmid#45059DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKif5c (Kif5c Rat)
TagsGFPExpressionMammalianMutationmotor domain aa1-559PromoterChicken Beta ActinAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-SypHluorin 2x (S2x)
Plasmid#37004DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSypHluorin 2x (Syp Rat)
TagsHAExpressionMammalianMutationTwo copies of pHluorin inserted at site 184-185PromoterCMVAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA 3.1 Flag-rat-PTP1B
Plasmid#35988DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertprotein tyrosine phosphatase 1B (Ptpn1 Rat)
TagsFlagExpressionMammalianPromoterCMVAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA4-Beclin1-(151-241)
Plasmid#24393DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBeclin1 (BECN1 Human)
TagsFLAGExpressionMammalianMutationAmino acids 151-241 are deleted. Delta CC mutant.Available sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
M77 pIRESpuro-GLUE-hDsh3
Plasmid#15102DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDsh3 (DVL3 Human)
TagsCBP tag, HA, and streptagExpressionMammalianAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro
Plasmid#213026PurposeExpresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2DepositorInsertHuman LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
Tags3xFlag (ORF2p)ExpressionMammalianPromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT
Plasmid#207832Purposetelomere specific nuclease to create double-strand breaks at telomeresDepositorInsertTRF1 (TERF1 Human)
UseLentiviralTagsFlag, DD, ER, mCherry and FokIExpressionMammalianPromoterTRE3GAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH21-BNA-Luc
Plasmid#187924PurposeExpresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertReporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
ExpressionMammalianPromoterRNA polymease I PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-nuclear-EKAR4
Plasmid#174439PurposeNuclear-targeted EKAR4.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnuclear-EKAR4
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi3-LgB91
Plasmid#134359PurposeNanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGαi3-LgB91 (GNAI3 Human)
TagsHAExpressionBacterial and MammalianPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-Calr-KDEL-IRES-Puromycin
Plasmid#134664PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorInsertcalreticulin (CALR Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 18-413PromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-MQ1(WT)-EGFP
Plasmid#89633PurposeTargeted CpG methylationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsite-specific DNA-methyltransferase SssI
UseCRISPRTags3*FLAG and 6*HisExpressionMammalianPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTH2
Plasmid#84452Purposeshuttle plasmid for sarA P1-CFP expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCFP Cerulean
UseShuttle vector e.coli-s.aureus, expression of sgf…Available sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by