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pAc5.1B-lambdaN-HA-DmCaf40-NFCVYAtoAAYYAF_W

Plasmid

#147829

Purpose

Insect Expression of DmCaf40-NFCVYAtoAAYYAF

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-NFCVYAtoAAYYAF (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMT-CTAP-PrP-SBP-DmCaf40_W

Plasmid

#147832

Purpose

Insect Expression of DmCaf40

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40 (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-NFCVYAtoAAYYAF_W

Plasmid

#147838

Purpose

Insect Expression of DmCaf40-NFCVYAtoAAYYAF

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-NFCVYAtoAAYYAF (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-R210AH213A_W

Plasmid

#147839

Purpose

Insect Expression of DmCaf40-R210AH213A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-R210AH213A (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4G_578-634-GB1_AG

Plasmid

#148744

Purpose

Bacterial Expression of DmeIF4G_578-634

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_578-634 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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UG76-pLYS2-GFP-PKCbeta

Plasmid

#184762

Purpose

Marker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

pLYS2-GFP-PKCbeta (PRKCB Budding Yeast, Human)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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DsRed -7e

Plasmid

#191765

Purpose

Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

DsRed

Tags

N terminal fusion of negatively charged polypetod…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70DA7-DadR/DadS-Pdadh-DadhABC(A2)

Plasmid

#191630

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulators DadR/DadS

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

Dopamine dehydroxylase

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadHR)-DadR-Pdadh-DadhABC(A2)

Plasmid

#191645

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulator DadR

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

Dopamine dehydroxylase

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.6RT4-Pct5.1-crRNA(hcdR)-RT(ΔhcdR)

Plasmid

#191646

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(hcdR-targeting spacer) hcdR-deleting repair template, used for hcdR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTV-Flnc-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174881

Purpose

Targeting vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Flnc

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Usp5-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174885

Purpose

Targeting vector for generating Usp5 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Usp5

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174886

Purpose

Targeting vector for generating Wnk1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Wnk1

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-3x-NLS-mScarlet-AMPKα2

Plasmid

#192452

Purpose

mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)

Tags

3x Nuclear localization signal (NLS) and mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 sf:em-blue-shift.2

Plasmid

#191919

Purpose

Encodes a GFP design, sf:em-blue-shift.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:em-blue-shift.2

Tags

His Tag

Expression

Bacterial

Mutation

T65S S72A T108I Y145F V150I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-1

Plasmid

#180400

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-1 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-2

Plasmid

#180401

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-2 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-3

Plasmid

#180402

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-3 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shStk16-2

Plasmid

#180392

Purpose

Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shStk16-2 (Stk16 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shStk16-3

Plasmid

#180393

Purpose

Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shStk16-3 (Stk16 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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