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Myc-Idax DBM

Plasmid

#68554

Purpose

This plasmid expresses full length IDAX containing a DNA binding mutation.

Depositor

Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

IDAX-DBM (Cxxc4 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

T162A, H164A and Q165A mutations

Promoter

EF1a

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pNICE HA-mSYD1A

Plasmid

#59361

Purpose

Expression plasmid for HA-tagged mSYD1A / Syde1 (HA-tag at N-terminus of mSYD1A)

Depositor

Peter Scheiffele

Article

Wentzel et al Neuron. 2013 Jun 19;78(6):1012-23. doi:

Insert

mSYD1A (Syde1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Intu

Plasmid

#65430

Purpose

For mammalian expression of EGFP-mouse Inturned

Depositor

Aimin Liu

Article

Zeng et al Dev Biol. 2010 Mar 15;339(2):418-28. doi

Insert

Inturned (Intu Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-deltaRING-LIN-41

Plasmid

#52718

Purpose

retroviral expression of human LIN-41 / TRIM71 containing a RING domain deletion and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 12-91

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX4T2-Ste7-EE

Plasmid

#52681

Purpose

expression of constitutively active Ste7 kinase in bacteria

Depositor

Benjamin Turk

Article

Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

Insert

Ste7 (STE7 Budding Yeast)

Tags

GST and Myc

Expression

Bacterial

Mutation

S359E/T363E constitutively active

Promoter

tac

Available Since

April 30, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Lin28B-mCCHC

Plasmid

#51375

Depositor

Narry Kim

Article

Heo et al Cell. 2009 Aug 21;138(4):696-708. doi: 1

Insert

Lin28B (LIN28B Human)

Tags

FLAG

Expression

Mammalian

Mutation

CCHC domain mutant (H137A, H159A)

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pCI-neo-FHM

Plasmid

#51871

Purpose

Expresses N-terminal FLAG-HA-tagged full length rat MIBP1 (cloned into pCI-neo)

Depositor

Kenshi Hayashi, Tomoko Tahira

Article

Iwashita et al J Biol Chem. 2012 Mar 23;287(13):9887-90

Insert

MIBP1 (Hivep2 Rat)

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
phpBCL2-FL(pA)

Plasmid

#42593

Depositor

Richard Lloyd

Article

Sherrill et al J Biol Chem. 2004 Jul 9;279(28):29066-74

Insert

hairpin - BCL2 5'UTR - firefly luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pGFH14A5

Plasmid

#39542

Depositor

Katharina Strub

Article

Lakkaraju et al Cell. 2008 May 2;133(3):440-51.

Insert

SRP14 (SRP14 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 96-100 changed to alanine

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only
Atg7_L (OZ557)

Plasmid

#28082

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting Atg7 (atg7 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
Atg7_R (OZ558)

Plasmid

#28083

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting Atg7 (atg7 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pSLIK-Venus-TmiR-G13-G12

Plasmid

#25742

Purpose

Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 and 12 miR-shRNA

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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GAG-PKB-pcDNA3

Plasmid

#24651

Depositor

Jim Woodgett

Article

Jones et al J Exp Med. 2000 May 15. 191(10):1721-34.

Insert

PKB (AKT1 Human)

Tags

GAG

Expression

Mammalian

Available Since

June 7, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1/nV5-DEST-Apr1

Plasmid

#20135

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pcDNA3.1/nV5-DEST-Arp1 (Actr1a Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
Vh-HA-FRB

Plasmid

#20225

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FRB (MTOR Human)

Tags

HA and Vh

Expression

Mammalian

Mutation

T2098L mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcR295/527

Plasmid

#17679

Depositor

David Shalloway

Article

Bagrodia et al Nature. 1991 Jan 10. 349(6305):172-5.

Insert

c-src K295R Y527F (SRC Chicken)

Expression

Mammalian

Mutation

Lys 295 changed to Arg. Tyr 527 changed to Phe.

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pGEX2TK-PDZ(HtrA2)

Plasmid

#16207

Depositor

L. Miguel Martins

Article

Martins et al J Biol Chem. 2003 Dec 5. 278(49):49417-2

Insert

HtrA2 (HTRA2 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 350–459

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
HOXB13 (human) Myc-tag-GST

Plasmid

#8683

Depositor

Corey Largman

Article

Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Insert

HOXB13 (HOXB13 Human)

Tags

GST and Myc

Expression

Bacterial

Mutation

makes protein by western

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1hisA cdc25c C330S

Plasmid

#10966

Depositor

Toren Finkel

Article

Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

Insert

cdc25c C330S (CDC25C Human)

Tags

6xHis

Expression

Mammalian

Mutation

C330S. Cysteine to Serine substitution at aa330.

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pBabe puro human Frataxin

Plasmid

#11505

Depositor

Ronald Kahn

Article

Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Frataxin (FXN Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only