3,614 results

1. pBP-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199202

   Purpose

   Gateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expression

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   split-intein-Gal4[21-881]-C-int
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHD-T2A-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199203

   Purpose

   To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[1-20]-N-int
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHD-T2A-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199204

   Purpose

   To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[21-881]-C-int
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. K560GFP

   Plasmid

   #129761

   Purpose

   Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Use

   Tags

   GFP and His6

   Expression

   Bacterial

   Mutation

   Truncation at 559

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMesh1*

   Plasmid

   #175591

   Purpose

   Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   Mesh1 (Mesh1 Fly)
   

   Use

   Tags

   Fused with a flexible GS linker to cerulean

   Expression

   Bacterial

   Mutation

   Codon optimized for translation in E.coli. AGGAGG…

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-Rab5

   Plasmid

   #31733

   Depositor

   Ron Vale

   Article

   Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

   Insert

   Rab5 (Rab5 Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   Actin

   Available since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pHD-DsRed-attP-w+

   Plasmid

   #80898

   Purpose

   Vector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.

   Depositor

   Kate O'Connor-Giles

   Inserts

   attP
   LoxP-3xP3-DsRed-LoxP
   GMR-white
   

   Use

   High copy, amp resistance

   Tags

   Expression

   Mutation

   Promoter

   3xP3 and GMR::hsp70

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

   Plasmid

   #217512

   Purpose

   KD Recombinase dependent conditional smGdP-10XOllas cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
   

   Use

   Tags

   smGdP-10XOllas

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBP-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199201

   Purpose

   Gateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expression

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   split-intein-Gal4[1-20]-N-int
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. dVGlut-Gal80

    Plasmid

    #52555

    Purpose

    Expresses Gal80 under vesicular glutamate transporter promoter

    Depositor

    Leslie Vosshall

    Insert

    VGlut-Gal80 (VGlut, GAL80 Budding Yeast, Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila Synthetic Core Promoter

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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