3,636 results

1. Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

   Plasmid

   #73164

   Purpose

   multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

   Depositor

   Bruce Edgar

   Article

   Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

   Insert

   mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)
   

   Tags

   mRFP1, GFP

   Expression

   Insect

   Mutation

   Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFD1-dU6:1gRNA

   Plasmid

   #49408

   Purpose

   expression of gRNA under control of the Drosophila U6:1 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-1:gRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6-1

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. 20XUAS IVS ChrimsonR mCherry_tr

   Plasmid

   #111546

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR_mCherry_trafficked
   

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Hy_pMT Laccase2 MCS Exon Vector

   Plasmid

   #69884

   Purpose

   Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   Laccase2 (stw Fly)
   

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCasper4-g-osk-TRICK

   Plasmid

   #71244

   Purpose

   oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

   Depositor

   Anne Ephrussi

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   oskar (osk Fly)
   

   Use

   P element-mediated germline transformation

   Tags

   12xPP7 and 6xMS2

   Expression

   Insect

   Promoter

   osk

   Available Since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc V5 dDispatched WT HA

   Plasmid

   #126405

   Purpose

   Expression of V5 dDispatched WT HA in Drosophila cells lines.

   Depositor

   Stacey Ogden

   Article

   Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

   Insert

   d-Dispatched1 (disp Fly)
   

   Tags

   HA

   Expression

   Insect

   Available Since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. KHBD00532

   Plasmid

   #39630

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG3629 (Dll Fly)
   

   Use

   Gateway donr vector

   Promoter

   none

   Available Since

   Aug. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pJC-repo1.9kb-TALE4-VP64-P10

   Plasmid

   #104612

   Purpose

   Expresses TALE4 under the control of a 1.9kb repo enhancer element

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   1.9kb repo enhancer element (repo Fly)
   

   Use

   TALEN

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC5.1-dCry-V5His

   Plasmid

   #136237

   Purpose

   Protein expression of D. melanogaster (Dm) cryptochrome with C-terminal V5 and His tags

   Depositor

   Steven Reppert, Aziz Sancar

   Article

   Zhu et al Curr Biol. 2005 Dec 6;15(23):R953-4. doi

   Insert

   DmCry
   

   Tags

   His and V5

   Expression

   Insect

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. PUC19_C_tag

    Plasmid

    #186659

    Purpose

    Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    C-terminal 3xFlag-3xHA Tag
    

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. dVGlut-Gal80

    Plasmid

    #52555

    Purpose

    Expresses Gal80 under vesicular glutamate transporter promoter

    Depositor

    Leslie Vosshall

    Insert

    VGlut-Gal80 (VGlut, GAL80 Budding Yeast, Fly)
    

    Expression

    Insect

    Promoter

    Drosophila Synthetic Core Promoter

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUASTLOTattB_VHH-GFP4::CD8::mCherry

    Plasmid

    #163917

    Purpose

    Expression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancers

    Depositor

    Markus Affolter

    Article

    Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

    Insert

    Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. LifeAct-MTED

    Plasmid

    #217498

    Purpose

    Expresses LifeAct-mScarlet-MTED for targeting fluorescent, MT depolymerizing peptide MTED to f-actin

    Depositor

    Erik Dent

    Article

    Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

    Insert

    LifeAct-mScarlet-MTED
    

    Expression

    Mammalian

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

    Plasmid

    #63167

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-cMyc"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMT-Hrp48-ADARcd-E488Q-V5

    Plasmid

    #139685

    Purpose

    Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

    Depositor

    Michael Rosbash

    Article

    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

    Insert

    Hrp48, ADAR (Adar Fly)
    

    Tags

    V5

    Expression

    Bacterial and Insect

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-KS-attB1-2-PT-SA-SD-1-GFP11

    Plasmid

    #172068

    Purpose

    Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP11x7
    

    Expression

    Insect

    Available Since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCB19

    Plasmid

    #194757

    Purpose

    EGFP-tagged GluRIIA-Construct for integration at attP sites

    Depositor

    Sven Dannhäuser

    Article

    Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

    Insert

    GluRIIA (GluRIIA Fly)
    

    Use

    Phic31-integration vector

    Tags

    EGFP-Tag

    Mutation

    none

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCB20

    Plasmid

    #194758

    Purpose

    ALFA-tagged GluRIIA-Construct for integration at attP sites

    Depositor

    Sven Dannhäuser

    Article

    Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

    Insert

    GluRIIA (GluRIIA Fly)
    

    Use

    Phic31-integration vector

    Tags

    ALFA-Tag

    Mutation

    none

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDmelOR-mRho.V5.mER.hOr47a

    Plasmid

    #126478

    Purpose

    Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Inserts

    Or47a (Or47a Fly)
    Orco (Orco Fly)
    

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens and codon optim…

    Promoter

    CMV

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFJ910

    Plasmid

    #44481

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, NeoR, MCS
    

    Use

    Multiple cloning site vector

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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