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Showing: 151 - 180 of 480 results

  1. pCIneo-hRL-Hmga2-3UTR-wt_R

    Plasmid
    #147351
    Purpose
    Mammalian Expression of Hmga2-3UTR-wt
    Depositor
    Elisa Izaurralde
    Insert
    Hmga2-3UTR-wt
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147351-plasmid-map-sequence-id-289798

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  2. pCIneo-hRL-Hmga-3UTR-m7_R

    Plasmid
    #147352
    Purpose
    Mammalian Expression of Hmga-3UTR-m7
    Depositor
    Elisa Izaurralde
    Insert
    Hmga-3UTR-m7
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147352-plasmid-map-sequence-id-289799

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  10. pSynSRE-T-Luc

    Plasmid
    #60444
    Purpose
    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.
    Depositor
    Timothy Osborne
    Article
    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.
    Insert
    HMG-CoA synthase promoter (-324/-225)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    60444-plasmid-map-sequence-id-266122

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  11. pSynSRE-Mut-T-Luc

    Plasmid
    #60490
    Purpose
    Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988
    Depositor
    Timothy Osborne
    Article
    Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.
    Insert
    HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
    Use
    Luciferase
    Tags
    Luciferase reporter
    Expression
    Mammalian
    Mutation
    Four point mutations (G->C or A->C) in the …
    Promoter
    Partial HMG-CoA synthase promoter (-324/-225) fus…
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    60490-plasmid-map-sequence-id-332535

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  12. pHAGE2-TetO-Chim-Pchi-Q61A

    Plasmid
    #231520
    Purpose
    Expresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation
    Depositor
    Ralf Jauch
    Article
    Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:
    Insert
    Pchi HMG Q61A flanked with N- and C-termini of mSox2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Point mutation at position 61 Q to A of the HMG d…
    Promoter
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    231520-plasmid-map-sequence-id-465593

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  15. eiABE

    Plasmid
    #227144
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain was inserted between TadA-8e and eIscBn driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-TadA-8e-linker-HMG-D-linker-nOgeuIscB-v3-bpNLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227144-plasmid-map-sequence-id-453987

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  16. hA3A*-eIscBn-D

    Plasmid
    #227147
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant and HMG-D domain was inserted between hA3A* and eIscBn driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-hA3A*-linker-HMG-D-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227147-plasmid-map-sequence-id-453978

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  17. pSMART-Mev1

    Plasmid
    #65815
    Purpose
    NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Inserts
    mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
    mvaS: hydroxymethylglutaryl-CoA synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    insulated mipAp from E. coli and insulated waaHp…
    Available since
    Sept. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65815-plasmid-map-sequence-id-111947

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  18. HBP1

    Plasmid
    #39092
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HBP1 (HBP1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pHS29

    Plasmid
    #171205
    Purpose
    bacterial expression for HIS purification of MEG-3 with mutations in the HMG-like motif
    Depositor
    Geraldine Seydoux
    Article
    Schmidt et al bioRxiv 2020.10.15.340570
    Insert
    meg-3 (meg-3 Nematode)
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    F705A,Y708A,Y713A, N725A
    Promoter
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171205-plasmid-map-sequence-id-335784

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  20. pHS33

    Plasmid
    #171202
    Purpose
    bacterial experssion of a GST-tagged MEG-3 C-terminus with mutations in the HMG-like motif
    Depositor
    Geraldine Seydoux
    Article
    Schmidt et al bioRxiv 2020.10.15.340570
    Insert
    meg-3 (meg-3 Nematode)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    aa545-862, F705A,Y708A,Y713A, N725A
    Promoter
    Available since
    Aug. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171202-plasmid-map-sequence-id-339776

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  21. pAAP21

    Plasmid
    #171207
    Purpose
    bacterial expression for HIS purification of the MEG-3 C-terminus with mutations in the HMG-like motif
    Depositor
    Geraldine Seydoux
    Article
    Schmidt et al bioRxiv 2020.10.15.340570
    Insert
    meg-3 (meg-3 Nematode)
    Use
    Tags
    8xHIS
    Expression
    Bacterial
    Mutation
    aa545-862, F705A,Y708A,Y713A, N725A
    Promoter
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171207-plasmid-map-sequence-id-335796

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Showing: 151 - 180 of 480 results