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We narrowed to 4,378 results for: crispr c plasmids

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Showing: 151 - 180 of 4378 results

  2. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

    Plasmid
    #135955
    Purpose
    Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Article
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Insert
    Prime editor v2 (PE2) N-terminal
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135955-plasmid-map-sequence-id-269512

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  3. PB-GFP-Gal8

    Plasmid
    #127191
    Purpose
    Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.
    Depositor
    Jordan Green
    Article
    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:
    Insert
    GFP-Gal8 (LGALS8 Human)
    Tags
    Gal8 is fused to the c-terminus of GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    127191-plasmid-map-sequence-id-248359

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  4. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid
    #114070
    Purpose
    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114070-plasmid-map-sequence-id-220511

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  7. pDD285

    Plasmid
    #66826
    Purpose
    mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.
    Insert
    mKate2-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans codon-optimized mKate2
    Expression
    Worm
    Available Since
    June 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. pUb-Cas9-EGFP_cU6:6-sgRNA

    Plasmid
    #190597
    Purpose
    The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.
    Depositor
    Claudia Rueckert
    Article
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    EGFP - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190597-plasmid-map-sequence-id-379952

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  23. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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  25. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid
    #190598
    Purpose
    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter
    Depositor
    Claudia Rueckert
    Article
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    mCherry - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190598-plasmid-map-sequence-id-379953

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  27. pCRISP_IS

    Plasmid
    #120425
    Purpose
    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.
    Depositor
    Tamás Fehér
    Article
    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:
    Insert
    CRISPR spacers targeting IS1, IS5, IS3
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    constitutive
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120425-plasmid-map-sequence-id-240993

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Showing: 151 - 180 of 4378 results