We narrowed to 2,478 results for: EFS
-
Plasmid#113686PurposeAn EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.DepositorInsertde-catalyzed SaCas9
UseAAV, CRISPR, and Cre/LoxTagsKRABAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189922PurposeAAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e
UseAAVMutationSaCas9 D10APromoterEFSAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2
Plasmid#223159PurposeExpression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKD‐SepRS-EFSep-5x tRNASep (B40 OTS)
Plasmid#52054PurposeUsed in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)DepositorInsertsSepRS
EF-Sep
5x tRNA Sep
ExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterPtacAvailable SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLY080b_AAV_EFS-4D5-CD8-28BBz (HER2 CAR)
Plasmid#192190PurposeHER2 CAR AAV vector PRODH2 KI (pLY080b)DepositorInsertHER2 CAR AAV vector PRODH2 KI (pLY080b) (ERBB2 Synthetic)
UseAAV; Mammalian expressionMutationNAAvailable SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-EFS-SpCas9-P2A-EGFP
Plasmid#99248Purposesingle-vector system with EGFP reporter for use in CROPseq knockout screensDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsEGFPExpressionMammalianPromoterEFSAvailable SinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657
Plasmid#212962PurposeLentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.DepositorInsertDR1_CasRx_non-targeting spacer
UseCRISPR and LentiviralTagsTagRFP657ExpressionMammalianPromoterU6Available SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657
Plasmid#212963PurposeLentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.DepositorInsertDR1_CasRx_GFP targeting spacer
UseCRISPR and LentiviralTagsTagRFP657ExpressionMammalianPromoterU6Available SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2
Plasmid#223149PurposeExpression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffoldDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM123: pAAV-EFS-CasRx-control presgRNA
Plasmid#166871PurposeAAV vector for expressing CasRx and control presgRNA for RNA-editingDepositorInsertsU6-control presgRNA
RfxCas13d
UseAAV and CRISPRTagsHA and NLSExpressionMammalianPromoterEFS and U6Available SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Sauri-U6-sgRNA scaffold
Plasmid#226921PurposeEncoding SauriABE8e and sgRNA cassette on a single AAVDepositorInsertTadA8e, nSauriCas9
UseAAVPromoterEFSAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLY101-RetroEFS-HER2-28BBz-PRODH2-WPRE
Plasmid#192202PurposeRetro-HER2CAR-PRODH2DepositorAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA
Plasmid#113685PurposeAn EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.DepositorInsertinverted de-catalyzed SaCas9
UseAAV, CRISPR, Cre/Lox, and Synthetic BiologyTagsNLS and VP64Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE
Plasmid#123360PurposeLentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.DepositorInsertEmpty LbCpf1 crRNA cloning cassette
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pM124: pAAV-EFS-CasRx-VEGFA presgRNA
Plasmid#166872PurposeAAV vector for expressing CasRx and human VEGFA targeting presgRNA for RNA-editingDepositorInsertsU6-VEGFA presgRNA
RfxCas13d
UseAAV and CRISPRTagsHA and NLSExpressionMammalianPromoterEFS and U6Available SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTE_02 - pLenti_EFS-Cas9-XTEN-FAN1-2A-BlastR
Plasmid#251998PurposeLentiviral mammalian expression of Cas9-FAN1 TruEditorDepositorInsertCas9-FAN1
UseCRISPR and LentiviralMutationNAAvailable SinceMarch 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTE_08 - pLenti_EFS-Cas9-XTEN-SPRTN-2A-BlastR
Plasmid#252004PurposeLentiviral mammalian expression of Cas9-SPRTN TruEditorDepositorInsertCas9-SPRTN
UseCRISPR and LentiviralMutationNAAvailable SinceMarch 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTE_17 - pLenti_EFS-Cas9-BARD1(32-117)-2A-BlastR
Plasmid#252013PurposeLentiviral mammalian expression of Cas9-BARD1(32-117) TruEditorDepositorInsertCas9-BARD1(32-117)
UseCRISPR and LentiviralMutationNAAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTE_16 - pLenti_EFS-Cas9-BLM(1-193)-2A-BlastR
Plasmid#252012PurposeLentiviral mammalian expression of Cas9-BLM(2-194) TruEditorDepositorInsertCas9-BLM(2-194)
UseCRISPR and LentiviralMutationNAAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTE_07 - pLenti_EFS-Cas9-XTEN-FEN1-2A-BlastR
Plasmid#252003PurposeLentiviral mammalian expression of Cas9-FEN1 TruEditorDepositorInsertCas9-FEN1
UseCRISPR and LentiviralMutationNAAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTE_06 - pLenti_EFS-Cas9-XTEN-RAD18-2A-BlastR
Plasmid#252002PurposeLentiviral mammalian expression of Cas9-RAD18 TruEditorDepositorInsertCas9-RAD18
UseCRISPR and LentiviralMutationNAAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTE_05 - pLenti_EFS-Cas9-XTEN-SLX1A-2A-BlastR
Plasmid#252001PurposeLentiviral mammalian expression of Cas9-SLX1A TruEditorDepositorInsertCas9-SLX1A
UseCRISPR and LentiviralMutationNAAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTE_04 - pLenti_EFS-Cas9-XTEN-ZNF146-2A-BlastR
Plasmid#252000PurposeLentiviral mammalian expression of Cas9-ZNF146 TruEditorDepositorInsertCas9-ZNF146
UseCRISPR and LentiviralMutationNAAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTE_03 - pLenti_EFS-Cas9-XTEN-BARD1-2A-BlastR
Plasmid#251999PurposeLentiviral mammalian expression of Cas9-BARD1 TruEditorDepositorInsertCas9-BARD1
UseCRISPR and LentiviralMutationNAAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTE_01 - pLenti_EFS-Cas9-XTEN-ZRANB3-2A-BlastR
Plasmid#251997PurposeLentiviral mammalian expression of Cas9-ZRANB3 TruEditorDepositorInsertCas9-ZRANB3
UseCRISPR and LentiviralMutationNAAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pKG3668_pGEEC578_pShip-EFS-rtTA-P2A-iRFP720-bGH
Plasmid#239741PurposePlasmid expressing a doxycycline-inducible transcriptional activatorDepositorInsertrtTA-P2A-iRFP720
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2437_pGEEC533_pShip-EFS-mRuby2-P2A-PuroR-bGH
Plasmid#239729PurposePlasmid expressing mRuby2 and PuroR with the EFS promoterDepositorInsertmRuby2-P2A-PuroR
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2309_pGEEC524_pShip-EFS-ZF10-NS3-p65-bGH
Plasmid#239722PurposePlasmid expressing a grazoprevir-inducible transcriptional activatorDepositorInsertZF10-NS3-p65
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA1
Plasmid#220117PurposeExpresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d start codon site by U6 promoterDepositorInsertTadA8e, nSauriCas9
UseAAVPromoterEFSAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA6
Plasmid#220118PurposeExpresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d intron6 5' splice site by U6 promoterDepositorInsertTadA8e, nSauriCas9
UseAAVPromoterEFSAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA7
Plasmid#220119PurposeExpresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoterDepositorInsertTadA8e, nSauriCas9
UseAAVPromoterEFSAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Nme2-U6-sgRNA scaffold
Plasmid#226933PurposeEncoding Nme2ABE8e and sgRNA cassette on a single AAVDepositorInsertTadA8e, nNme2Cas9
UseAAVPromoterEFSAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-SaKKH-U6-sgRNA scaffold
Plasmid#226935PurposeEncoding SaKKHABE8e and sgRNA cassette on a single AAVDepositorInsertTadA8e, nSaKKHCas9
UseAAVPromoterEFSAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
p5E-EFS (EF1a core) (JDW 1319)
Plasmid#224474PurposeGateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.DepositorInsertEFS promoter
ExpressionMammalianAvailable SinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E EFS-rtTA/rtTA3 (JDW 1214)
Plasmid#224533PurposeA Gateway compatible 3' entry clone containing the Human EFS promoter driving rtTA-3rd generation transactivatorDepositorInsertrtTA
UseGateway cloningPromoterEFSAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1297-AAV-EFSNC-dCjCas9-HP1b(79-173)
Plasmid#223142PurposeExpression of truncated HP1b with dCjCas9 and empty gRNA scaffoldDepositorInsertHP1b (CBX1 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 79-173PromoterEF1aAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1295-AAV-EFSNC-dCjCas9-HP1a(72-177)
Plasmid#223140PurposeExpression of truncated HP1a with dCjCas9 and empty gRNA scaffoldDepositorInsertHP1a (CBX5 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 72-177PromoterEF1aAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1305-AAV-EFSNC-dSaCas9-HP1a(72-177)
Plasmid#223150PurposeExpression of truncated HP1a with dSaCas9 and empty gRNA scaffoldDepositorInsertHP1a (CBX5 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 72-177PromoterEF1aAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173)
Plasmid#223152PurposeExpression of truncated HP1b with dSaCas9 and empty gRNA scaffoldDepositorInsertHP1b (CBX1 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 79-173PromoterEF1aAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9
Plasmid#223163Purpose2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA ScaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)Available SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only