We narrowed to 1,992 results for: RP1

1. pDONR221 NLRP1 A54T

   Plasmid

   #166815

   Purpose

   Gateway pDONR vector encoding NLRP1 with the patient mutation A54T (Zhong et al., 2016) (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   A54T

   Promoter

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDB.His.MBP.3C NLRP1 UPA WT

   Plasmid

   #164010

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pICE TRP1-URA3

   Plasmid

   #122894

   Purpose

   TRP1-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pICE TRP1-HphMx

   Plasmid

   #122901

   Purpose

   TRP1-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21-Drp1(deltaIB)

   Plasmid

   #73041

   Purpose

   Bacterial expression of mouse Drp1 isoform b (minus residues 514-602) with C-terminal His-tag and PreScission protease cleavage site

   Depositor

   David Chan

   Article

   Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

   Insert

   Drp1(deltaIB) (Dnm1l Mouse)
   

   Use

   Tags

   6xHis-tag and PreScission Protease site

   Expression

   Bacterial

   Mutation

   Deleted amino acids 514-602

   Promoter

   T7

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PYDonly-NLRP11.MYC

   Plasmid

   #131198

   Purpose

   mammalian expression

   Depositor

   Thomas Kufer

   Article

   Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

   Insert

   PYD domain of NLRP11 (NLRP11 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   contains only the pyrin domain (amino acids 1-128)

   Promoter

   pCMV

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFA6a-BIO-TRP1

   Plasmid

   #26881

   Depositor

   Peter Kaiser

   Article

   Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

   Insert

   BIO-tag
   

   Use

   Pcr tagging yeast

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-RGS18HIS-TRP1

   Plasmid

   #26884

   Depositor

   Peter Kaiser

   Article

   Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

   Insert

   18xHis tag
   

   Use

   Pcr tagging yeast

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. T7-His-Arp11(1–417)

   Plasmid

   #51400

   Purpose

   expression of full-length mouse Arp11 under T7 promoter

   Depositor

   Trina Schroer

   Article

   Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

   Insert

   Arp11 (Actr10 Mouse)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pRS424 Prp8 WT TRP1

    Plasmid

    #234120

    Purpose

    The N-terminus of Prp8 was restored thereby destroying the NheI site and correcting the ORF

    Depositor

    Aaron Hoskins

    Insert

    Prp8
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PB-PARP1-del.p119K120S–eGFP

    Plasmid

    #211554

    Purpose

    A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Piggybac

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    deletion of amino acids 119K 120S

    Promoter

    CMV

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDN0520 (pDEST, TRP1)

    Plasmid

    #210481

    Purpose

    pDEST vector with TRP1 marker for gene expression in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Destination vector for gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-hARFRP1(T31N)-HA

    Plasmid

    #206401

    Purpose

    Mammalian expression of hARFRP1(T31N)

    Depositor

    Kazuhisa Nakayama, Hye-Won Shin

    Article

    Man et al J Biol Chem. 2011 Apr 1;286(13):11569-78

    Insert

    ARFRP1 (ARFRP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T31N

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-hARFRP1(Q79L)-HA

    Plasmid

    #206400

    Purpose

    Mammalian expression of hARFRP1(Q79L)

    Depositor

    Kazuhisa Nakayama, Hye-Won Shin

    Article

    Man et al J Biol Chem. 2011 Apr 1;286(13):11569-78

    Insert

    ARFRP1 (ARFRP1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q79L

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET3-ARFRP1-His6

    Plasmid

    #201882

    Purpose

    Used for bacterial expression of human protein ARFRP1

    Depositor

    Richard Kahn

    Insert

    ARFRP1 (ARFRP1 Human)
    

    Use

    Tags

    6xHis (GSGSGSHHHHHH)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSCM167.2-Venus-VF-hSFRP1

    Plasmid

    #189541

    Purpose

    Protein expression of SFRP1 in mammalian cells.

    Depositor

    Haian Fu

    Insert

    secreted frizzled related protein 1 (SFRP1 Human)
    

    Use

    Tags

    VF

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti-sgLrp1b#2/Cre

    Plasmid

    #173642

    Purpose

    Expresses a Lrp1b-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Lrp1b (Lrp1b Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-sgLrp1b#1/Cre

    Plasmid

    #173641

    Purpose

    Expresses a Lrp1b-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Lrp1b (Lrp1b Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28_6xHis-PARP1delZn1-L713F

    Plasmid

    #173937

    Purpose

    Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn1-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 1-96; L713F, V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28_6xHis-PARP1delZn3-L713F

    Plasmid

    #173934

    Purpose

    Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn3-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 214-373; L713F, V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28_6xHis-PARP1-G871L

    Plasmid

    #174798

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-G871L (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G871L, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28_6xHis-PARP1-Zn2

    Plasmid

    #174805

    Purpose

    Bacterial expression of isolated PARP1 Zn2 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Zn2 (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28_6xHis-PARP1-Zn3

    Plasmid

    #174806

    Purpose

    Bacterial expression of isolated PARP1 Zn3 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Zn3 (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28_6xHis-PARP1-A870L

    Plasmid

    #174797

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A870L (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A870L, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28_6xHis-PARP1-K893I

    Plasmid

    #174799

    Purpose

    Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-K893I (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K893I, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-DEST42 -SSRP1

    Plasmid

    #170439

    Purpose

    Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

    Depositor

    Joan Conaway

    Article

    Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

    Insert

    ssrp1 (Ssrp Fly)
    

    Use

    Tags

    6xHis and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR221 NLRP1 A59P

    Plasmid

    #166816

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation A59P (Herlin et al., 2020) (no stop codon)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    A59P

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR221 Ub-NLRP1 L1215

    Plasmid

    #166828

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR221 Ub-NLRP1 S1213

    Plasmid

    #166827

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR221 NLRP1 T755N

    Plasmid

    #166820

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation T755N (Drutman et al., 2019) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    T755N

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR221 NLRP1 R726W

    Plasmid

    #166819

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation R726W (Grandemange et al., 2017) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    R726W

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR221 Ub-NLRP1 L1218

    Plasmid

    #166829

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. dTAG NLRP1 P1214R

    Plasmid

    #166826

    Purpose

    dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P1214R

    Promoter

    hPGK

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX6P1-GCN4cc-Erp1CT

    Plasmid

    #73921

    Purpose

    GST fusion- coiled coil- Erp1 cytoplasmic tail

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Erp1 CT
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGEX6P1- Mst7-Erp1CT

    Plasmid

    #73920

    Purpose

    GST fusion- Erp1 cytoplasmic tail

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Erp1 CT
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SSRP1 G9.2 gRNA

    Plasmid

    #90904

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SSRP1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SSRP1 (Guide Designation G9.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SSRP1 G10.2 gRNA

    Plasmid

    #90905

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SSRP1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SSRP1 (Guide Designation G10.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pT2-cryR;nrp1bP1Egfp

    Plasmid

    #90164

    Purpose

    contains nrp1b specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    nrp1bP1Egfp
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDEST40-ARFRP1-V5-His6

    Plasmid

    #67450

    Purpose

    Mammalian expression of hArfrp with C-term V5 and His6 tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARFRP1 (ARFRP1 Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

40. pDSHA-ARFRP1-Myc

    Plasmid

    #67366

    Purpose

    Mammalian expression of hArfrp with C-term Myc tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARFRP1 (ARFRP1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only