We narrowed to 693 results for: emd

Showing: 201 - 240 of 693 results

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ELK1_pet28a

Plasmid

#131647

Purpose

Bacterial expression of ETS gene ELK1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELK1 (ELK1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a His tag-FRB-Venus C

Plasmid

#100980

Purpose

Encodes half of a protein delivery sensor (His-FRB-Venus C) for expression in and purification from Bacteria

Depositor

Heinrich Leonhardt

Article

Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

Insert

His-FRB-Venus C

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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ELF4_pet28a

Plasmid

#131661

Purpose

Bacterial expression of ETS gene ELF4

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF4 (ELF4 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21b-LaG9(S151C)

Plasmid

#172490

Purpose

Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

Depositor

Siegfried Musser

Article

Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

Insert

LaG9(S151C)

Tags

6xHis, PPX cleavage site, and pelB leader sequenc…

Expression

Bacterial

Mutation

S151C

Promoter

T7

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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SPI1_pet28a

Plasmid

#131653

Purpose

Bacterial expression of ETS gene SPI1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPI1 (SPI1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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ETV3L_pet28a

Plasmid

#131666

Purpose

Bacterial expression of ETS gene ETV3L

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV3L (ETV3L Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET14b_SG

Plasmid

#59737

Purpose

Expresses SH3-GUK domains of human ZO-1

Depositor

Alan Fanning

Article

Lye et al J Biol Chem. 2010 Apr 30;285(18):13907-1

Insert

zonula occludens-1 (TJP1 Human)

Tags

HIS tag and thrombin clvg

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
SRS-MTBD(26/19) AB(monomer), wt

Plasmid

#22381

Depositor

Ron Vale

Article

Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

Insert

Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein

Tags

myc

Expression

Bacterial

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pET15b/EYFP-GgVcl 1-851

Plasmid

#46264

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

Insert

Vcl 1-851 (VCL Chicken)

Tags

EYFP and His

Expression

Bacterial

Mutation

aa 1-851 (aka Vh) head domain

Promoter

T7

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pACYC-Skp-hLYZ

Plasmid

#83924

Purpose

Co-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
lysozyme (LYZ Human)

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b/GgVcl V1-851 (aka Vh)

Plasmid

#46172

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

Insert

Vcl 1-851 (VCL Chicken)

Tags

His

Expression

Bacterial

Mutation

aa 1-851 (aka Vh) head domain

Promoter

T7

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
SRS-MTBD(26/19)ux, dimer, no myc

Plasmid

#22382

Depositor

Ron Vale

Article

Carter et al Science. 2008 Dec 12. 322(5908):1691-5.

Insert

Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein

Tags

His

Expression

Bacterial

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-2H12Matry

Plasmid

#221187

Purpose

His6-tagged cdGreen-Matry expressed from T7 promoter (Internal Lab ID: UJ11309)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen-Matry

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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SMT215

Plasmid

#134818

Purpose

SMT205 with DHFR-FLAG-tag, western blot

Depositor

Tanja Kortemme

Article

Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

Inserts

Dihydrofolate reductase
Thymidylate synthase

Tags

FLAG

Expression

Bacterial

Mutation

Silent mutations to eliminate internal restrictio…

Promoter

T7 and TET

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SMT101

Plasmid

#134815

Purpose

Dual expression of DHFR and TYMS, in vivo assays

Depositor

Tanja Kortemme

Article

Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

Inserts

Dihydrofolate reductase
Thymidylate synthase

Expression

Bacterial

Mutation

Silent mutations to eliminate internal restrictio…

Promoter

T7

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SMT201

Plasmid

#134816

Purpose

SMT101 with TET promoter for TYMS, in vivo assays

Depositor

Tanja Kortemme

Article

Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

Inserts

Dihydrofolate reductase
Thymidylate synthase

Expression

Bacterial

Mutation

Silent mutations to eliminate internal restrictio…

Promoter

T7 and TET

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b/GgVcl 1-258 A50I mutation

Plasmid

#46175

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-258 A50I (VCL Chicken)

Tags

His

Expression

Bacterial

Mutation

aa 1-258 (VD1) A50I mutation; talin binding defic…

Promoter

T7

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
p40deltaPX (w/out aa1-135)

Plasmid

#19019

Depositor

Michael Yaffe

Article

Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

Insert

p40phox (NCF4 Human)

Tags

His

Expression

Bacterial

Mutation

aa136-148 (PX domain, truncated)

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
Mucin MUC1-5TR-pET15b

Plasmid

#174694

Purpose

Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

Depositor

Jean Gariepy

Article

Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

Insert

MUC1-TR

Tags

hexahistidine (HIS) tag

Expression

Bacterial

Mutation

none

Promoter

T7 Promoter

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pet-15b-ultraID

Plasmid

#172879

Purpose

expresses ultraID in E. coli

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

ultraID

Tags

myc

Expression

Bacterial

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28C-mCherry-YTHDF2

Plasmid

#204406

Purpose

E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

YTHDF2 (YTHDF2 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-Cas9-His

Plasmid

#98158

Purpose

Purification of Cas9 protein

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

Insert

NLS-Cas9-NLS

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-TS2126 RNA Ligase

Plasmid

#119941

Purpose

Express TS2126 RNA ligase in E. coli

Depositor

Takashi Ito

Article

Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

Insert

TS2126 RNA ligase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKSJ376

Plasmid

#103065

Purpose

Expresses colicin E1 with R domain deletion and ImmE1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Inserts

colicin E1 immunity protein (pColE1_26, imm E. coli)
cea/colicin E1

Expression

Bacterial

Mutation

R (receptor-binding domain) deletion in colicin E…

Promoter

T7 lac (from pET) and T7 lac (from pET52-b)

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pet-15b-microID

Plasmid

#172881

Purpose

expresses microID in E. coli

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

microID

Tags

myc

Expression

Bacterial

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ELF1_pet28a

Plasmid

#131649

Purpose

Bacterial expression of ETS gene ELF1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF1 (ELF1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)::MBP-NL3F10H

Plasmid

#141291

Purpose

MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

MBP-NLUC3F10H

Use

Luciferase

Tags

Flag (x3), His (x10), and MBP

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FLI1_pet28a

Plasmid

#131641

Purpose

Bacterial expression of ETS gene FLI1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

FLI1 (FLI1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ERG_pet28a

Plasmid

#131642

Purpose

Bacterial expression of ETS gene ERG

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ERG (ERG Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
JT113-pETDuet1-(R)-hREV3L

Plasmid

#64872

Purpose

bacterial expression of human REV3L

Depositor

Richard Wood

Article

Tomida et al Nucleic Acids Res. 2015 Jan;43(2):1000-1

Insert

REV3L (REV3L Human)

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pWUR703

Plasmid

#53082

Purpose

inducible TtAgoDM (D478A,D546A) expression

Depositor

John van der Oost

Article

Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

Insert

TtAgo

Tags

Strep-II tag

Expression

Bacterial

Mutation

D478A D546A

Promoter

T7

Available Since

May 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
His-drebrin

Plasmid

#40362

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only
pET28C-SARS-CoV-2-Nucleocapsid

Plasmid

#204402

Purpose

E. coli expression of full-length SARS-CoV-2 nucleocapsid

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

SARS-CoV-2 nucleocapsid (N SARS-CoV-2)

Tags

6x-His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDJSVT86

Plasmid

#108965

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

Depositor

Daniel Slade

Article

Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

Type

Empty backbone

Tags

OmpA 1-27, 6His

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28C-mCherry-DDX4LCD

Plasmid

#204408

Purpose

E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

DDX4(1-236) (DDX4 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ETV5_pet28a

Plasmid

#131639

Purpose

Bacterial expression of ETS gene ETV5

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV5 (ETV5 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ELK3_pet28a

Plasmid

#131651

Purpose

Bacterial expression of ETS gene ELK3

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELK3 (ELK3 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FEV_pet28a

Plasmid

#131668

Purpose

Bacterial expression of ETS gene FEV

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

FEV (FEV Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ETV1_pet28a

Plasmid

#131644

Purpose

Bacterial expression of ETS gene ETV1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV1 (ETV1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
drebrin-His

Plasmid

#40363

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Tags

His and T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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We narrowed to 693 results for: emd