Addgene

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PCR4-beta-globin::lacZ-H11

Plasmid

#227000

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains human beta-globin minimal promoter)

Depositor

Guillaume Andrey

Article

Darbellay et al Nat Commun. 2024 Jun 6;15(1):4820. doi:

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

beta-globin

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-mEGFP-CCR4 isoform A (HK52)

Plasmid

#206439

Purpose

CCR4 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al bioRxiv 2022.03.04.482790

Insert

CCR4 (twin Fly)

Use

Tags

mEGFP

Expression

Insect

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-CCR4 isoform A-mCherry-PH (EB7)

Plasmid

#206473

Purpose

CCR4 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al bioRxiv 2022.03.04.482790

Insert

CCR4 (twin Fly)

Use

Tags

mcherry PH

Expression

Insect

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4-4xmut-dsRNAres_R

Plasmid

#147425

Purpose

Insect Expression of DmCCR4-4xmut-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4-4xmut-dsRNAres (twin Fly)

Use

Tags

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4_168-552-D412AN414A_R

Plasmid

#147427

Purpose

Insect Expression of DmCCR4_168-552-D412AN414A

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_168-552-D412AN414A (twin Fly)

Use

Tags

Expression

Insect

Mutation

4 silent mutations and 1 non silent mutations com…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4-L42EI44E-dsRNAres_R

Plasmid

#147429

Purpose

Insect Expression of DmCCR4-L42EI44E-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4-L42EI44E-dsRNAres (twin Fly)

Use

Tags

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTR-450 tNGFR-P2A-CXCR4

Plasmid

#186078

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CXCR4 (CXCR4 Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11

Plasmid

#211942

Purpose

dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)

Depositor

Evgeny Kvon

Article

Hollingsworth et al bioRxiv 2023.12.10.570890

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

HSP68

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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PCR4-HBGP::mCherry-SI-HBGP::eGFP-H11

Plasmid

#216394

Purpose

dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct (human beta-globin promoter) into the H11 locus (separated by a synthetic insulator)

Depositor

Evgeny Kvon

Article

Hollingsworth et al bioRxiv 2023.12.10.570890

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Human beta-globin

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4_1-166-L42EI44E-dsRNAres_R

Plasmid

#147426

Purpose

Insect Expression of DmCCR4_1-166-L42EI44E-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_1-166-L42EI44E-dsRNAres (twin Fly)

Use

Tags

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101113

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to CXCR4

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

CXCR4-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101114

Purpose

Encodes dCas9(N) fused to CXCR4

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

CXCR4-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9noSsrA

Plasmid

#102285

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

removed ssrA tag

Promoter

pTet

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1

Plasmid

#89961

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9HF1

Use

Tags

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pdCas9-sgRNA-RFP

Plasmid

#166005

Purpose

Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

Depositor

Martin Buck

Article

Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pC1-FGB (FlincG2)

Plasmid

#49204

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Article

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Insert

FlincG2 (PRKG1 GFP, Bovine)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2014

Availability

Academic Institutions and Nonprofits only

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(E6) CMV-p18 C/EBPalpha

Plasmid

#25881

Depositor

Ormond MacDougald

Article

Erickson et al J Biol Chem. 2001 May 11. 276(19):16348-

Insert

p18 C/EBPalpha (Cebpa Mouse)

Use

Tags

Expression

Mammalian

Mutation

N-terminal truncation, contains only CR4 and bZIP…

Promoter

Available since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsNot6-L34EI36E_S

Plasmid

#147508

Purpose

Mammalian Expression of HsNot6-L34EI36E

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot6-L34EI36E (CNOT6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-159-557_AF

Plasmid

#148657

Purpose

Bacterial Expression of HsNot6_159-557

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6_159-557 (CNOT6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-D491A_AF

Plasmid

#148659

Purpose

Bacterial Expression of HsNot6-D491A

Depositor

Elisa Izaurralde

Article

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6-D491A (CNOT6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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