We narrowed to 164,853 results for: Gene

1. pTE5: pFuGW-SSX1p- GAL4BD-4X3CLpro synthetic general CS-VP16AD-SV40NLS

   Plasmid

   #240460

   Purpose

   a synthetic transcription factor (synTF) that contains 4 tandem synthetic general 3CLpro cleavage site (CS).

   Depositor

   Lior Nissim

   Article

   Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

   Inserts

   SSX1p
   GAL4BD-4X3CLpro synthetic general CS-VP16AD-SV40NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

   Plasmid

   #60815

   Purpose

   dCas9 with single cysteine residue (M1C) for site-specific labelling

   Depositor

   Jennifer Doudna

   Article

   O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

   Insert

   dCas9 M1C D10A C80S H840A C574S
   

   Use

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   M1C D10A C80S H840A C574S

   Promoter

   Available Since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDest17_Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene B protein LCD (1-136)

   Plasmid

   #235001

   Purpose

   Bacterial expression of N-terminally 6His tagged Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene B protein LCD

   Depositor

   Tanja Mittag

   Insert

   Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene B protein (LOC129490717 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57-ITR2-H1-GFP sgRNA Target 3- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

   Plasmid

   #210739

   Purpose

   Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 3

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   Sp Cas9 (cas9 S pyogenes)
   Sp sgRNA GFP Target 3
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   Promoter

   H1 and eCMV

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57-ITR2-H1-GFP sgRNA Target 1- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

   Plasmid

   #210737

   Purpose

   Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 1

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   Sp Cas9 (cas9 S pyogenes)
   Sp sgRNA GFP Target 1
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   Promoter

   H1 and eCMV

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-ITR2-H1-GFP sgRNA Target 2- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

   Plasmid

   #210738

   Purpose

   Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 2

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   Sp Cas9 (cas9 S pyogenes)
   Sp sgRNA GFP Target 2
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   Promoter

   H1 and eCMV

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-ITR2-H1-GFP sgRNA Target 4- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

   Plasmid

   #210740

   Purpose

   Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 4

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   Sp Cas9 (cas9 S pyogenes)
   Sp sgRNA GFP Target 4
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   Promoter

   H1 and eCMV

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pttq18

   Plasmid

   #69122

   Purpose

   regulated expression of genes in Escherichia coli

   Depositor

   Michael Stark

   Article

   Stark et al Gene. 1987;51(2-3):255-67.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVU/myc

   Plasmid

   #24179

   Depositor

   Lars Ittner

   Insert

   Chloramphenicol resistance gene (CmR) - ccdB gene
   

   Use

   Lentiviral; Gateway

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pDGM3B

    Plasmid

    #110809

    Purpose

    contains the AAV3B cap genes, AAV2 rep genes, and also adenovirus helper genes

    Depositor

    David Russell

    Article

    Khan et al Nat Protoc. 2011 Apr;6(4):482-501. doi:

    Insert

    AAV3B cap genes, AAV2 rep genes, adenovirus helper genes
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAW32

    Plasmid

    #110225

    Purpose

    integration of loxP site directly upstream of gene start site - contains S. pombe nmt1 promoter

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAW33

    Plasmid

    #110226

    Purpose

    integration of loxP site directly upstream of gene start site - contains S. pombe nmt41 promoter

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAW34

    Plasmid

    #110227

    Purpose

    integration of loxP site directly upstream of gene start site - contains S. pombe nmt81 promoter

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJQ200mp18

    Plasmid

    #78500

    Purpose

    Suicide vector for gene replacement

    Depositor

    Michael Hynes

    Article

    Quandt et al Gene. 1993 May 15;127(1):15-21.

    Type

    Empty backbone

    Use

    Suicide vector for gene replacement

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAW41

    Plasmid

    #110228

    Purpose

    integration of loxP site a distance upstream of gene start site

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Insert

    ura4+ (ura4 Fission Yeast)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    See depositor comments below.

    Promoter

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAW5

    Plasmid

    #110221

    Purpose

    Cre-expression plasmid

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAW8

    Plasmid

    #110222

    Purpose

    Cre-expression plasmid for cassette exchange

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJQ200uc1

    Plasmid

    #78502

    Purpose

    Suicide vector for gene replacement, modified MCS to maximise utility of insert restriction sites.

    Depositor

    Michael Hynes

    Article

    Quandt et al Gene. 1993 May 15;127(1):15-21.

    Type

    Empty backbone

    Use

    Suicide vector for gene replacement

    Tags

    none

    Expression

    Mutation

    Promoter

    none

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

19. SA-betageo

    Plasmid

    #21709

    Depositor

    Philippe Soriano

    Article

    Friedrich et al Genes Dev. 1991 Sep . 5(9):1513-23.

    Insert

    SAbgeo
    

    Use

    Mouse gene trapping

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAW8_6XFLAG

    Plasmid

    #110235

    Purpose

    Cre/Lox C-terminal tagging construct encoding 6 copies of the FLAG epitope

    Depositor

    Tony Carr

    Article

    Watson et al Gene. 2008 Jan 15;407(1-2):63-74

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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