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Plasmid#136584PurposeExpresses an inducible short hairpin targeting human NRF2 sequenceDepositorInsertshNRF2v1 (NFE2L2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
tet_pLKO.1_puro_shNRF2 #2
Plasmid#136585PurposeExpresses an inducible short hairpin targeting human NRF2 sequenceDepositorInsertshNRF2v2 (NFE2L2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2_3xflag_NRF2
Plasmid#136527PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIKDepositorInsertNRF2 (NFE2L2 Human)
UseEntry vector for gateway cloningTags3x FLAGExpressionMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_BirA_CDKN1A
Plasmid#136538PurposeLentiviral expression vector for an inducible BirA-CDKN1ADepositorInsertBirA-CDKN1A (CDKN1A )
UseLentiviral; Destinatioin vector for gateway cloni…TagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_BirA_NRF2
Plasmid#136539PurposeLentiviral expression vector for an inducible BirA-NRF2DepositorInsertBirA-NRF2 (NFE2L2 Human)
UseLentiviral; Destination vector for gateway cloningTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO.1_puro_shJUND #1
Plasmid#136581PurposeExpresses an inducible short hairpin targeting human JUND sequenceDepositorInsertshJUNDv1 (JUND Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2_BirA_CDKN1A
Plasmid#136523PurposeUsed as a donor vector to clone into pSLIKDepositorInsertCDKN1A (CDKN1A Human)
UseEntry vector for gateway cloningTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO.1_puro_shJUND #2
Plasmid#136583PurposeExpresses an inducible short hairpin targeting human JUND sequenceDepositorInsertshJUNDv2 (JUND Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSLIK_neo_3xflag_CHEK2
Plasmid#136536PurposeLentiviral expression vector for an inducible 3xflag-tagged CHEK2DepositorInsertCHEK2 (CHEK2 Human)
UseLentiviral; Destinatioin vector for gateway cloni…Tags3x FLAGExpressionMammalianMutationPromoterAvailable sinceJune 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRC2_p53DD
Plasmid#136520PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIKDepositorInsertp53DD (Trp53 Mouse)
UseEntry vector for gateway cloningTags3x FLAGExpressionMutationThis construct was created by PCR from the pBabe …PromoterAvailable sinceOct. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2_3xflag_CHEK2
Plasmid#136526PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIKDepositorInsertCHEK2 (CHEK2 Human)
UseEntry vector for gateway cloningTags3x FLAGExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSLIK Zeo p53DD
Plasmid#136534PurposeLentiviral expression vector for an inducible p53DDDepositorInsertp53DD (Trp53 Mouse)
UseLentiviral; Destinatioin vector for gateway cloni…TagsExpressionMammalianMutationThis construct was created from the pBabe p53DD (…PromoterAvailable sinceFeb. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE_hygro_mRFP1_NRF2(C506S, L4A)
Plasmid#136580Purposemammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut)DepositorInsertNRF2 (C506S, Leuzip mut) (NFE2L2 Human)
UseRetroviralTagsN-mRFP1-FLAGExpressionMammalianMutationThe C506S mutation (C513S in the actual sequence …PromoterAvailable sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
G13-CASE
Plasmid#168127PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F125/D126 within GNA13PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gi1-CASE
Plasmid#168120PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A121/E122 within GNAI1PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gi3-CASE
Plasmid#168122PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A114/E115 within GNAI1PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gq-CASE
Plasmid#168125PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F124/E125 within GNAQPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_3xflag_NRF2(RR)
Plasmid#136535PurposeLentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)DepositorInsertNRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
UseLentiviral; Destinatioin vector for gateway cloni…Tags3x FLAGExpressionMammalianMutationThis plasmid is developed by mutating 3 base pair…PromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_p53(R280K)_V5
Plasmid#136540PurposeLentiviral expression vector for an inducible p53(R280K)-V5DepositorInsertp53(R280K) (TP53 Human)
UseLentiviral; Destinatioin vector for gateway cloni…TagsV5ExpressionMammalianMutationp53(R280K)V5 was cloned from pLenti6-p53-R280K-V5…PromoterAvailable sinceJune 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRC2_3xflag_NRF2(RR)
Plasmid#136522PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIKDepositorInsertNRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
UseEntry vector for gateway cloningTags3x FLAGExpressionMutationThis plasmid is developed by mutating 3 base pair…PromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only