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Showing: 101 - 120 of 273 results

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E1_pENTR_L1-L4_NI_G1333-DddA11'-C

Plasmid

#199799

Purpose

ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_NN_G1333-DddA11'-C

Plasmid

#199800

Purpose

ptpTALECD_v2 vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
E3_pENTR_L3-L2_HD_G1333-DddA11'-N

Plasmid

#199801

Purpose

ptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
E3_pENTR_L3-L2_NG_G1333-DddA11'-N

Plasmid

#199802

Purpose

ptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
E3_pENTR_L3-L2_NI_G1333-DddA11'-N

Plasmid

#199803

Purpose

ptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
E3_pENTR_L3-L2_NN_G1333-DddA11'-N

Plasmid

#199804

Purpose

ptpTALECD_v2 vector assembly, Entry clone 3 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Plant J. 2023 Aug;115(4):1151-1162. doi:

Insert

C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11'), and a uracil glycosylase inhibitor

Use

Talen

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP in pDBEcR-pIND(SP1)/Bla

Plasmid

#201557

Purpose

Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.

Depositor

Randy Poon

Article

Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

Insert

DBEcR and EGFP

Use

Unspecified; Sleeping beauty transposon

Tags

DBEcR-myc and FLAG-EGFP

Expression

Mammalian

Mutation

Promoter

EF1a and pIMD(SP1)

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP in pDBEcR-pIND(SP1)-W/Bla

Plasmid

#201558

Purpose

Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

Depositor

Randy Poon

Article

Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

Insert

DBEcR and EGFP

Use

Unspecified; Sleeping beauty transposon

Tags

DBEcR-myc and FLAG-EGFP

Expression

Mammalian

Mutation

Promoter

EF1a and pIND(SP1)

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP in pDBEcR-pIND(SP1)-DD/Bla

Plasmid

#201559

Purpose

Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD and DBEcR (3xmyc-tagged); blasticidin resistance.

Depositor

Randy Poon

Article

Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

Insert

DBEcR and EGFP

Use

Unspecified; Sleeping beauty transposon

Tags

DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD

Expression

Mammalian

Mutation

Promoter

EF1a and pIND(SP1)

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP in pDBEcR-pIND(SP1)-DD-W/Bla

Plasmid

#201560

Purpose

Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

Depositor

Randy Poon

Article

Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

Insert

DBEcR and EGFP

Use

Unspecified; Sleeping beauty transposon

Tags

DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD

Expression

Mammalian

Mutation

Promoter

EF1a and pIND(SP1)

Availability

Academic Institutions and Nonprofits only

Enlarge
BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

Plasmid

#136688

Purpose

Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.

Depositor

Colin Garvie

Article

Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

Insert

Beclin-1 (BECN1 Human)

Use

Tags

Trigger Factor

Expression

Bacterial

Mutation

DNA optimized for E. coli expression w/ three mut…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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p031 pCS2 End-1 ORF

Plasmid

#11030

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

End-1 (end-1 Nematode)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIS8-3_DL121

Plasmid

#171953

Purpose

Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Insert

Fusion of ecDHFR and asLOV2 at position 121 of DHFR

Use

Tags

8-His tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_DL121_WTTS

Plasmid

#171954

Purpose

Selection vector for chimeric fusion of ecDHFR and asLOV2.

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Inserts

Fusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase

Use

Tags

8-His tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pTJV1Sc-rpoB

Plasmid

#166978

Purpose

Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Inserts

Ec86 Retron (ECD_00831 )
lambda bet

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23101 and VanCC

Availability

Academic Institutions and Nonprofits only

Enlarge
pDBEcR/Bla

Plasmid

#201556

Purpose

Plasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.

Depositor

Randy Poon

Article

Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

Insert

DBEcR

Use

Unspecified; Sleeping beauty transposon

Tags

Myc

Expression

Mammalian

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

Enlarge
p026 pCS2-EnR

Plasmid

#11028

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

engrailed (en Fly)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Engrailed repressor domain (bases encoding amino …

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-RL-ZEB1

Plasmid

#35535

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB1 (ZEB1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-RL-ZEB1 200bmutx5

Plasmid

#35537

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB1 (ZEB1 Human, Budding Yeast)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

5 mutations in miR200b seed regions of 3'UTR…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
KHBD00332

Plasmid

#34455

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1765 (EcR Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only