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pCMV-HA-mIFITM3-K104A

Plasmid

#58403

Purpose

Expresses murine IFITM3-K104A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysine 104 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-CUL1 K720A

Plasmid

#19939

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 K720A (CUL1 Human)

Use

Tags

HA2

Expression

Mammalian

Mutation

CUL1 with a NEDD8 modification mutant

Promoter

Available since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
pUB6-CDC34-HA (pRKB261)

Plasmid

#99145

Purpose

Mammalian expression construct to express HA-tagged CDC34

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

CDC34 (CDC34 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

pTight

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-KLF5-his

Plasmid

#40904

Depositor

Jin-Tang Dong

Article

Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

Insert

Kruppel-like factor 5 (KLF5 Human)

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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CAV1-K*R-HA

Plasmid

#27765

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1, lysine null mutant (CAV1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

All lysines mutated to arginines (K*R)

Promoter

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pRK5-Flag-HA-Merlin

Plasmid

#27104

Depositor

Filippo Giancotti

Article

Li et al Cell. 2010 Feb 19. 140(4):477-90.

Insert

Merlin (Nf2 Mouse)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pRK5-Merlin-Flag-HA

Plasmid

#27103

Depositor

Filippo Giancotti

Article

Li et al Cell. 2010 Feb 19. 140(4):477-90.

Insert

Merlin (Nf2 Mouse)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24,83,88,104A

Plasmid

#58408

Purpose

Expresses murine IFITM3-K24,83,88,104A with an N-terminal HA tag in mammalian cells (Lysine-less nonubiquitinated mutant)

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 24, 83, 88, and 104 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K83,88,104A

Plasmid

#58405

Purpose

Expresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 83, 88, and 104 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
HIS-HA-CMYC P53

Plasmid

#233738

Purpose

expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

Depositor

Albert Keung

Article

Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

Insert

HIS-HA-CMYC-p53 (TP53 Human)

Use

Tags

HIS-HA-CMYC

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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HA-RIPLET ΔPRY/SPRY

Plasmid

#218516

Purpose

RIPLET lacking C-terminal PRY/SPRY domain in pcDNA3.1

Depositor

Sun Hur

Article

Cadena et al Cell. 2019 May 16;177(5):1187-1200.e16.

Insert

RIPLET delta PRY/SPRY domain (RNF135 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-HA-mIFITM3-D92N

Plasmid

#58412

Purpose

Expresses murine IFITM3-D92N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Apartate 92 to Asparagine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24,88,104A

Plasmid

#58406

Purpose

Expresses murine IFITM3-K24,88,104A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 24, 88, and 104 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-N2A

Plasmid

#58410

Purpose

Expresses murine IFITM3-N2A with an N-terminal HA tag in mammalian cells (mutates possible glycosylation site)

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Asparagine 2 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-H136T

Plasmid

#58413

Purpose

Expresses murine IFITM3-H136T with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Histidine 236 to Threonine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24,83,104A

Plasmid

#58407

Purpose

Expresses murine IFITM3-K24,83,104A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 24, 83, and 104 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-S38N

Plasmid

#58411

Purpose

Expresses murine IFITM3-S38N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 38 to Asparagine

Promoter

CMV IE

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24,83,88A

Plasmid

#58404

Purpose

Expresses murine IFITM3-K24,83,88A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 24, 83, and 88 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pLX209-neo-HA-Ub-T2A-Tet3G

Plasmid

#202436

Purpose

Bicistronic expression of HA-tagged Ubiquitin (HA-Ubiquitin) and Tet-On 3G transactivator (Tet3G)

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

Ubiquitin

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBE2QL1-Strep/HA

Plasmid

#124666

Purpose

Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Use

Tags

2xStrep/HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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