pSN-A112C-CP8B1
Plasmid
#191240
Purpose
Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
Depositor
Insert
nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
Use
Tags
nuclease A from S. aureus fused to the poly-Leu T…
Expression
Bacterial
Mutation
Changed Alanine 112 to Cysteine in SN, and predic…
Promoter
T7 promoter
Available since
Dec. 21, 2022
Availability
Academic Institutions and Nonprofits only
