We narrowed to 6,266 results for: wus;

1. pFastBac HTB DPP9S S730A

   Plasmid

   #169946

   Purpose

   Purification of catalytically-dead DPP9S from insect cells

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   HIS-TEV

   Expression

   Insect

   Mutation

   S730A

   Promoter

   polyhedrin

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV Flag dPDZ-PTEN (1-400)

   Plasmid

   #22232

   Depositor

   Hong Wu

   Article

   Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

   Insert

   PTEN (Pten Mouse)
   

   Use

   Tags

   Flag

   Expression

   Bacterial and Mammalian

   Mutation

   PDZ binding domain deleted. Contains amino acids …

   Promoter

   Available Since

   March 19, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized

   Plasmid

   #166834

   Purpose

   Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   TEV-GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 LIC 6A CARD8-FLAG S297A

   Plasmid

   #169949

   Purpose

   Mammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S297A

   Promoter

   CMV

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDB.His.MBP.3C NLRP1 CARD WT

   Plasmid

   #164008

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-mScarlet-SARS-CoV-2-SL1 alone

   Plasmid

   #191485

   Purpose

   Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone

   Depositor

   Hao Wu

   Article

   Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

   Insert

   SARS-CoV-2 5' UTR SL1 alone (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDB.His.MBP.3C CARD8 UPA-CARD WT

   Plasmid

   #164021

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac HTB DPP9S D808N

   Plasmid

   #169947

   Purpose

   Purification of catalytically-dead DPP9S from insect cells

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   HIS-TEV

   Expression

   Insect

   Mutation

   D808N

   Promoter

   polyhedrin

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGL3Basic-Mdm2-T1

   Plasmid

   #32365

   Depositor

   Hong Wu

   Article

   Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

   Insert

   Mdm2 promoter (Mdm2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pLV-mScarlet-SARS-CoV-2-5'UTRΔSL1

    Plasmid

    #191484

    Purpose

    Lentiviral expression of SARS-CoV-2 5' UTR ΔSL1

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    SARS-CoV-2 5' UTR ΔSL1 (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE

    Plasmid

    #166798

    Purpose

    Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    LL100EE

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-mScarlet-MAVS-5'UTR

    Plasmid

    #184639

    Purpose

    Fluorescent reporter for mammalian cells: Expression of mScarlet with MAVS 5'UTR

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    mScarlet
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 2, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pGL3Basic-Mdm2-T2

    Plasmid

    #32366

    Depositor

    Hong Wu

    Article

    Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

    Insert

    Mdm2 promoter (Mdm2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pET28a-DDX43-K292A

    Plasmid

    #134575

    Purpose

    Express human DDX43 mutant (K292A, ATPase dead) in E. coli

    Depositor

    Yuliang Wu

    Article

    Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

    Insert

    DDX43-K292A (DDX43 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    full-length (K292A)

    Promoter

    T7

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3Basic-Mdm-P1

    Plasmid

    #32367

    Depositor

    Hong Wu

    Article

    Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

    Insert

    Mdm2 promoter (Mdm2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1 6B CARD8 UPA-CARD-mCherry WT

    Plasmid

    #164011

    Purpose

    Mammalian expression vector encoding CARD8 UPA-CARD with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUASTP2

    Plasmid

    #13843

    Depositor

    Ting Wu

    Article

    Bateman et al Genetics. 2006 Jun . 173(2):769-77.

    Insert

    mini-white (w Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET29a-hNABP1-C

    Plasmid

    #128311

    Purpose

    Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-6P-1-INTS3-N

    Plasmid

    #128416

    Purpose

    Express INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    INTS3 (INTS3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-terminal region (1–513 aa)

    Promoter

    tac promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-6P-1-INTS3-C

    Plasmid

    #128417

    Purpose

    Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    INTS3 (INTS3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    INTS3 C-terminal region (514-1042 aa)

    Promoter

    Tac promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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