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We narrowed to 30,297 results for: uros

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pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

Plasmid

#112927

Purpose

CRISPR-a reporter assay lentiviral vector (gTetO)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMK444 (EF1-OsTIR1(F74G) Puro)

Plasmid

#214374

Purpose

EF1-OsTIR1(F74G) (PiggyBac)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

EF1-OsTIR1(F74G)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1 promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti PGK V5-LUC Puro (w543-1)

Plasmid

#19360

Purpose

3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Lentiviral and Luciferase

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-PEmax-P2A-Puro

Plasmid

#228890

Purpose

Lentiviral delivery and expression of PEmax protein with puromycin selection cassette.

Depositor

Francisco J Sánchez-Rivera

Article

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Inserts

PEmax
Puromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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GFP shRNA tet pLKO puro

Plasmid

#163017

Purpose

Control tet-inducible shRNA targeting eGFP

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-Myr-Flag-AKT1

Plasmid

#15294

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AKT1 (AKT1 Human)

Use

Retroviral

Tags

Myr-Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO RasV12 Puro (w119-1)

Plasmid

#22262

Depositor

Eric Campeau

Insert

HRAS G12V (HRAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

G12V mutant

Promoter

Available since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-LbNOX-FLAG

Plasmid

#218631

Purpose

This plasmid contains LbNOX under doxycycline inducible control.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

LbNOX

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

Plasmid

#102798

Purpose

Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Inserts

sgRNA
EGFP

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

H231L

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VP64-Puro

Plasmid

#99371

Purpose

3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-VP64-T2A-Puro

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-hGFAP-NEUROD1-T2A-mCherry

Plasmid

#178582

Purpose

Expression of NEUROD1 and mCherry under the human GFAP promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

NEUROD1 (NEUROD1 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

Plasmid

#112926

Purpose

CRISPR-a reporter assay lentiviral vector (empty gRNA control)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(empty), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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TRIPZ-CTID195-GSQ-SUMO1nc-PURO

Plasmid

#208035

Purpose

Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

Depositor

James Sutherland, Rosa Barrio

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

SUMO1nc (SUMO1 Human)

Use

Lentiviral

Tags

Myc, C-terminal Split-TurboID

Expression

Mutation

Q94P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

Plasmid

#155307

Purpose

Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6, EF1a

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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TRIPZ-CTID195-GSQ-UBnc-PURO

Plasmid

#208037

Purpose

Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

Depositor

James Sutherland, Rosa Barrio

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

Ubnc (UBC Human)

Use

Lentiviral

Tags

Myc, C-terminal Split-TurboID

Expression

Mutation

L73P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pL-U6-sgRNA-SFFV-Puro-P2A-EGFP

Plasmid

#175037

Purpose

Lentiviral delivery of sgRNA. Expresses PuroR and eGFP from SFFV promoter.

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Sp sgRNA scaffold

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FMR1 isoform1-sh1Resistant

Plasmid

#225462

Purpose

Express mEGFP-fusion protein of FMR1 isoform 1

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 isoform 1 (FMR1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mRuby3-IRES-Puro

Plasmid

#155231

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mRuby sensor

Use

Lentiviral

Tags

mRuby3

Expression

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SlugABE-NNG-P2A-puro

Plasmid

#214354

Purpose

Expresses SlugABE-NNG and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugABE-NNG and puromycin resistance genes

Use

Tags

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)

Plasmid

#225752

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-SauriCas9-KKH-Puro

Plasmid

#135966

Purpose

Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV[TetOn]-Puro-TRE3G>mAscl1

Plasmid

#198755

Purpose

Vector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-puro-tetON-shOGDH 2497

Plasmid

#110942

Purpose

Tet-inducible shRNA targeting OGDH

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shOGDH (OGDH Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

H1/TO

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-puro-tetON-shOGDH 1714

Plasmid

#110941

Purpose

Tet-inducible shRNA targeting OGDH

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shOGDH (OGDH Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

H1/TO

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-puro-tetON-shOGDH 2956

Plasmid

#110943

Purpose

Tet-inducible shRNA targeting OGDH

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

shOGDH (OGDH Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

H1/TO

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FLAG-2xSTREP-CDKN1B-Puro

Plasmid

#172611

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDKN1B (CDKN1B Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti CMVtight eGFP Puro (w771-1)

Plasmid

#26431

Purpose

2nd generation lentiviral vector

Depositor

Eric Campeau

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral; Tet-on advanced vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 puro shRNA beta-catenin

Plasmid

#18803

Purpose

3rd gen lentiviral vector for knocking down beta-catenin gene expression

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

b-catenin shRNA (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
N-Myc-53BP1 D1521A pLPC-Puro

Plasmid

#19837

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Oct 19. ():.

Insert

p53 Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

changed Asp1521 to Ala

Promoter

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only
pLenti CMVtight Puro DEST (w768-1)

Plasmid

#26430

Depositor

Eric Campeau

Type

Empty backbone

Use

Lentiviral; Tet-on advanced destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pIVTR(T7A)-PuroR-T2A-mScarlet

Plasmid

#172301

Purpose

In vitro transcription of PuroR-T2A-mScarlet synthetic construct

Depositor

Patrik Verstreken

Insert

PuroR-T2A-mScarlet

Use

Other

Tags

Expression

Mutation

Promoter

T7 promoter A-initiating

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-FLAG-puro-PTPIP51(1-470)

Plasmid

#170536

Purpose

Expresses PTPIP51(1-470) in human HeLa cells after transfection

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only
HA-HIF1alpha P402A/P564A-pBabe-puro

Plasmid

#19005

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 402 to Alanin…

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pNKX2-5-T2A-GFP-PGK-Puro

Plasmid

#203607

Purpose

Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

Depositor

Matthew Birket

Article

Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

Insert

NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)

Use

Human targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
METTL3 shRNA 2 tet pLKO puro

Plasmid

#162984

Purpose

Tet-inducible shRNA targeting human METTL3 #2

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

methyltransferase like 3 (METTL3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti PGK mKate2-OGT WT (Puro)

Plasmid

#154290

Purpose

Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, WT)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x FLAG and mKate2

Expression

Mammalian

Mutation

WT

Promoter

PGK

Available since

Dec. 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

Plasmid

#171594

Purpose

Strong human ACE2 abundance from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

ACE2 (ACE2 Human)
mCherry
H2A
Pac

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-EFIa-Id1-PGK-puro

Plasmid

#107735

Purpose

Expresses Id1 in mammalian cells

Depositor

Alexandre Colas

Article

Cunningham et al Genes Dev. 2017 Aug 9. pii: gad.300400.1

Insert

Id1 (T Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti CMV/TO eGFP Puro (w159-1)

Plasmid

#17481

Purpose

3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Pan-Neurofascin (extracellular) scFv [A12/18]

Plasmid

#190485

Purpose

Mammalian Expression of Pan-Neurofascin (extracellular) scFV. Derived from hybridoma A12/18.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant scFV (Nfasc Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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