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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,445 results for: MB

Showing: 1181 - 1200 of 1445 results

  6. pETDUET-GST AO7RE ∆238-245

    Plasmid
    #139318
    Purpose
    GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria
    Depositor
    Allan Weissman
    Article
    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3
    Insert
    AO7 aa 126-258 (RNF25 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    AO7 cloned into the first multiple cloning site o…
    Promoter
    T7 promoter-1
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    139318-plasmid-map-sequence-id-275576

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  12. pIFU002

    Plasmid
    #174299
    Purpose
    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Insert
    Spike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HA and cMyc
    Expression
    Yeast
    Mutation
    N343Q, N501Y
    Promoter
    Available since
    Sept. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    174299-plasmid-map-sequence-id-342196

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  13. pIFU001

    Plasmid
    #174298
    Purpose
    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Insert
    Spike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HA and cMyc
    Expression
    Yeast
    Mutation
    N343Q, E484K
    Promoter
    Available since
    Sept. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    174298-plasmid-map-sequence-id-342193

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  14. pGG442

    Plasmid
    #165616
    Purpose
    Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
    Use
    CRISPR
    Tags
    3x HA, SV40 NLS, Zif268, and c-Myc-like NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165616-plasmid-map-sequence-id-330958

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  15. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

    Plasmid
    #91819
    Purpose
    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD
    Depositor
    Chris Hill
    Article
    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1
    Insert
    RPO21 (RPO21 Budding Yeast)
    Use
    Tags
    10xHis
    Expression
    Yeast
    Mutation
    5xGly linker, PreScission Protease site (LEVLFQGP…
    Promoter
    Endogenous Rpb1 promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    91819-plasmid-map-sequence-id-176494

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  16. CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

    Plasmid
    #91820
    Purpose
    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD
    Depositor
    Chris Hill
    Article
    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1
    Insert
    RPO21 (RPO21 Budding Yeast)
    Use
    Tags
    10xHis
    Expression
    Yeast
    Mutation
    5xGly linker, PreScission Protease site (LEVLFQGP…
    Promoter
    Endogenous Rpb1 promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    91820-plasmid-map-sequence-id-181105

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Showing: 1181 - 1200 of 1445 results