We narrowed to 1,631 results for: Pts

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HA-dora[A]_rescue_construct

Plasmid

#190640

Purpose

Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-G120E-del133-155-V5-SBP-MBP_V

Plasmid

#147767

Purpose

Mammalian Expression of HsSmg5-G120E-del133-155

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-G120E-del133-155 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-G120E-siRNAres-V5-SBP-MBP_S

Plasmid

#147470

Purpose

Mammalian Expression of HsSmg5-G120E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg5-G120E-siRNAres (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTH862-CEN-slowmaxRLuc/minCFLuc

Plasmid

#115371

Purpose

Expresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferase

Depositor

Tobias von der Haar

Inserts

Firefly Luciferase (codon minimised)
Gcn4 5'-UTR + Renilla luciferase (codon optimised)

Expression

Yeast

Mutation

All codons have been changed for the most favoura…

Promoter

ADH1 and TDH3

Available Since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a - Ec-ClpX D144L

Plasmid

#111514

Purpose

Expresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

ClpX

Tags

His tag

Expression

Bacterial

Mutation

Aspartatic acid 144 to Leucine

Promoter

T7

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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p198 Dync1i2.F (Ex1a)

Plasmid

#26448

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform F (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
pmGFP-ADAR1-p150

Plasmid

#117927

Purpose

ADAR1-p150 expression plasmid.

Depositor

Kumiko Ui-Tei

Article

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Insert

ADAR1-p150 (ADAR Human)

Tags

myc-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pmGFP-ADAR1-p110

Plasmid

#117928

Purpose

ADAR1-p110 expression plasmid.

Depositor

Kumiko Ui-Tei

Article

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Insert

ADAR1-p110 (ADAR Human)

Tags

myc-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES2_EGFP_KCNQ1

Plasmid

#173161

Purpose

For mammalian expression of KCNQ1 and creating variants

Depositor

Al George

Article

Vanoye et al Circ Genom Precis Med. 2018 Nov;11(11):e

Insert

KCNQ1 (KCNQ1 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRF+423Dux4

Plasmid

#21625

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

Double homeobox, chr 4 (DUX4 Human)

Use

Luciferase

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
Lentiviral-S-tdTomato

Plasmid

#112579

Purpose

Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

Depositor

Jeffrey Rothstein

Article

Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

Insert

NLS-tdTomato-NES

Use

Lentiviral

Tags

nuclear export sequence and nuclear localization …

Expression

Mammalian

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-PRDX1

Plasmid

#38086

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

PRDX1 peroxiredoxin 1 (PRDX1 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

Plasmid

#187588

Purpose

Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

NLS

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

Plasmid

#182925

Purpose

Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Type

Empty backbone

Tags

APEX2 and FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term

Plasmid

#182926

Purpose

Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Type

Empty backbone

Tags

APEX2 and FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_003

Plasmid

#192505

Purpose

For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

Depositor

Neville Sanjana

Article

Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

Insert

hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE

Use

Lentiviral

Promoter

hU6

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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canxTM-mEmerald (cytosolic)

Plasmid

#186962

Purpose

Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

CANX (CANX Human)

Tags

mEmerald

Expression

Mammalian

Mutation

Only express calnexin (482-end) and add ER signal…

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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mEmerald(luminal)-canxTM

Plasmid

#186961

Purpose

Expresses mEmerald connected with the N-terminus of Calnexin transmembrane domain.

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

CANX (CANX Human)

Tags

mEmerald

Expression

Mammalian

Mutation

Only express calnexin (482-end) with ER signal pe…

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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PGK EGFP-PPM1H-WT

Plasmid

#200084

Purpose

Mammalian expression of wild-type PPM1H

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

PPM1H (PPM1H Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-mkgDUX4

Plasmid

#21156

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX4 (DUX4 Human)

Expression

Bacterial and Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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