We narrowed to 4,616 results for: pcr
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Plasmid#71486PurposeContains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.DepositorInsertloxP-hyg-loxP cassette
UseSource of loxp-hyg-loxp for pcr amplification.ExpressionBacterialAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM343
Plasmid#71487PurposeContains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.DepositorInsertloxP-hyg-cat-loxP
UseSource of loxp-hyg-cat- loxp for pcr amplificatio…ExpressionBacterialAvailable SinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSWt1-Dest
Plasmid#59764PurposePCR template for amplifying the attL1-em7-kanr-attL2-pA cassetteDepositorInsertattL1-em7-kanR-attL2-pA
Available SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNR1D2-donor
Plasmid#112314PurposeCRISPR donor plasmid to tag human transcription factor NR1D2 with GFPDepositorInsertNR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
UseCRISPRMutationhg38:3:23977483:A>T, hg38:3:23977515:A>G, h…Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRP2350
Plasmid#127580PurposeTagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and Hyg resistance geneDepositorInsertPtetO7.1_Flagtag_8Glycine linker
UseSynthetic Biology; Pcr based taggingTagsFlag tag, 8glycine linkerAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPkan-S
Plasmid#41017PurposePCR template for en passant (two-step Red-mediated) mutagenesisDepositorInsertKana_I-SceI
Available SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRP2375
Plasmid#127581PurposeTagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and NAT resistance geneDepositorInsertPtetO7.1_Flagtag_8Glycine linker
UseSynthetic Biology; Pcr based taggingTagsFlag tag, 8glycine linkerAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
mUAV
Plasmid#102680PurposeMobius Assembly Universal Acceptor Vector, it converts amplified PCR fragments into standard, exchangeable partsDepositorInsertJ23106:B0034:amilCP:rrnBT1-T7TE
UseSynthetic BiologyAvailable SinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPkan-S2
Plasmid#61601PurposePCR template for en passant (two-step Red-mediated) mutagenesisDepositorInsertKana_I-SceI
Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pATF4-donor
Plasmid#113805PurposeCRISPR donor plasmid to tag human transcription factor ATF4 with GFPDepositorInsertATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
UseCRISPRMutationrs140428747Available SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA CRU HISMX
Plasmid#131164PurposePCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutantDepositorTypeEmpty backboneTagsCRUExpressionYeastAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8739
Plasmid#126905PurposeTemplate for PCR amplification of XdCrtYBDepositorInsertXdCrtYB
UseSynthetic BiologyAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8740
Plasmid#126906PurposeTemplate for PCR amplification of XdCrtIDepositorInsertXdCrtI
UseSynthetic BiologyAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8741
Plasmid#126907PurposeTemplate for PCR amplification of XdCrtEDepositorInsertXdCrtE
UseSynthetic BiologyAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
XE85 Delta Beta-catenin in GFP
Plasmid#16835DepositorInsertDelta beta-catenin GFP (ctnnb1.L Frog)
UseXenopus expressionTagsGFPMutationThese PCR fragments have the first 138 bp after t…Available SinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCfB8818
Plasmid#126909PurposeTemplate for PCR amplification of HMG1from Yarrowia lipolyticaDepositorInsertYlHMG1
UseSynthetic BiologyAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pattB-aubergine-ADH-gf
Plasmid#69448Purposegenomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead AubDepositorInsertaubergine (aub Fly)
TagsNAExpressionInsectMutationchr2L:10,998,626(−)T>GPromoteraubergineAvailable SinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
TAL-3TA4
Plasmid#41472DepositorInsert3TA4
UsePcr cloning vectorAvailable SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL-4GA1
Plasmid#41504DepositorInsert4GA1
UsePcr cloning vectorAvailable SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only