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pBP-Bba_K822000

Plasmid

#74094

Purpose

L-Lactate inducible promoter from E. coli MG1655

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_K822000 (L-Lactate promoter - E. coli MG1655)

Use

Synthetic Biology

Expression

Bacterial

Promoter

NA

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMSP2N2

Plasmid

#29520

Depositor

Stephen Sligar

Article

Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

Insert

MSP2N2

Tags

7-His tag

Expression

Bacterial

Mutation

tandem MSP; His-tag on N-terminus followed by spa…

Available Since

May 3, 2011

Availability

Academic Institutions and Nonprofits only

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pFREE_Cm

Plasmid

#92052

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Promoter

pRhamBAD and ptet

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRL1342

Plasmid

#70691

Purpose

RSF1010-based vector with extensive polylinker, and resistance to Cm and Em. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.

Depositor

Peter Wolk

Article

Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

Type

Empty backbone

Use

Bacterial shuttle vector

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pIM1708-surT-PsurP-agaN-pNW33N

Plasmid

#44009

Depositor

Ichiro Matsumura

Article

Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

Insert

surT-PsurP-agaN

Expression

Bacterial

Mutation

Deleted EcoRI site (synonymous change, C to T at …

Available Since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-teLuc c-myc

Plasmid

#100026

Purpose

Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

teLuc, a NanoLuc mutant

Tags

c-myc

Expression

Mammalian

Mutation

D19S, D85N, C164H

Promoter

CMV

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAFi-dHdrA

Plasmid

#184907

Purpose

carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cm-sgRNA-dCas9

Use

CRISPR

Expression

Bacterial

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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CRBN-NanoLuc Vector

Plasmid

#238749

Purpose

Express CRBN-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

CRBN (CRBN Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLVpuro-CMV-N-3xFLAG

Plasmid

#123223

Purpose

N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLVpuro-CMV-N-EGFP

Plasmid

#122848

Purpose

N-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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