We narrowed to 19,077 results for: REV;

Showing: 11981 - 12000 of 19077 results

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pCA528 SNX15 MIT residues 263-342

Plasmid

#180625

Purpose

Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SNX15 (SNX15 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 263-342

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , F124D, residues 108-200

Plasmid

#180629

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200, F124D

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , L177D, residues 108-200

Plasmid

#180634

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200, L177D

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 USP8, residues 1-147

Plasmid

#180636

Purpose

Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

USP8 (USP8 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-147

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 USP54, residues 731-878

Plasmid

#180637

Purpose

Bacterial expression for USP54 MIT domain, residues 731-878. Has N-terminal HIS-SUMO tag. Internal ID:WISP20-85

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

USP54 (USP54 Human)

Tags

HIS-SUMO

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET16B VPS4A, residues 1-84

Plasmid

#180638

Purpose

Bacterial expression for VPS4A MIT domain residues 1-84. Has N-terminal HIS-TAG. Internal ID: WISP05-43

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

VPS4A (VPS4A Human)

Tags

His tag

Expression

Bacterial

Mutation

Residues 1-84

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165, V18D

Plasmid

#180612

Purpose

Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165, V18D mutation

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165, F98D

Plasmid

#180613

Purpose

Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165, F98D mutation

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

Plasmid

#197426

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMVQA

Plasmid

#197894

Purpose

pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

Depositor

Thomas Klimkait

Article

Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

Insert

HIV-1 3' end (HXB2: 9045 till poly-A(10)

Expression

Bacterial

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGA-red-mini

Plasmid

#196338

Purpose

Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Golden gate assembly backbone

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD

Plasmid

#199447

Purpose

Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal

Depositor

Baohui Chen

Article

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

dSaCas9-10xGCN4

Use

Lentiviral

Expression

Mammalian

Promoter

human ubiquitin C (UBC)

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pJR035

Plasmid

#198821

Purpose

For tissue specific RNA-seq

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::rpl-22HA-SL2-mKate::let-858 3'UTR

Expression

Worm

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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tet pLKO.1-shCse1l v1 puro

Plasmid

#192342

Purpose

Lentiviral expression vector for an inducible shCse1L v1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

shCse1l v1 (Cse1l Budding Yeast)

Use

Lentiviral

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mNudcd3 - 1

Plasmid

#198501

Purpose

lentiviral stable expression of mNudcd3 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mNudcd3 (Nudcd3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mNudcd3 - 2

Plasmid

#198502

Purpose

lentiviral stable expression of mNudcd3 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mNudcd3 (Nudcd3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mRab12-2

Plasmid

#198476

Purpose

lentiviral stable expression of mRab12 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mouse Rab12 (Rab12 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mRab12-1

Plasmid

#198475

Purpose

lentiviral stable expression of mRab12 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mouse Rab12 (Rab12 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4G_578-675-GB1_AD

Plasmid

#148504

Purpose

Bacterial Expression of DmeIF4G_578-675

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_578-675 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4G_601-634-GB1_AD

Plasmid

#148505

Purpose

Bacterial Expression of DmeIF4G_601-634

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_601-634 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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