We narrowed to 9,520 results for: try
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Plasmid#160412PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC2) for HySyn system fused to mCherryDepositorInsertpDONR221 HyCal2::mCherry
UseOtherTagsmCherryAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
[GFP][IRES][Bla]
Plasmid#97194PurposeVector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.DepositorInsertsbeta-Lactamase
GFP
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-mos::NG
Plasmid#138958PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutation8 bp that are in the region that encodes the cata…PromoterUASAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-004
Plasmid#78691PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-003
Plasmid#78690PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r
Plasmid#162958PurposeGateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteinsDepositorInserttriple FLAG epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-SGTAP no-pA
Plasmid#82599Purpose3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purificationDepositorInsertSBP-TEV-2x protein G
PromoterNoneAvailable SinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-013
Plasmid#78701PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
C234-E08: attB2r-3xFLAG-attB3
Plasmid#162912PurposeGateway attB2r/attB3 entry clone for construction of C-terminal triple-FLAG epitope tagged fusion proteinsDepositorInserttriple FLAG epitope tag
UseSynthetic BiologyAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E44: attB5r-SNAP-nostop-attB1r
Plasmid#162962PurposeGateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteinsDepositorInsertSNAP tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only