We narrowed to 823 results for: Anti-CRISPR

1. pM-ABE

   Plasmid

   #196292

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding ABE in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Mutation

   See depositor comments below

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. ScFv-2ERT2-VPH

   Plasmid

   #120556

   Purpose

   Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to 2 tandem ERT2, VP64, P65 and HSF1

   Depositor

   Yu Wang

   Article

   Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

   Insert

   scFvGCN4, GB1, ERT2, VP64, P65 and HSF1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ScFv-2ERT2-V

   Plasmid

   #120553

   Purpose

   3rd gen transfer vector. Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP, 2 tandem ERT2 and VP64.

   Depositor

   Yu Wang

   Article

   Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

   Insert

   scFvGCN4, sfGFP, GB1, ERT2, VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLiP-G2-RacE

   Plasmid

   #188966

   Purpose

   L-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdown

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   dCas9
   sgRNA: gttagacgctgattacatggactagg
   racE
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNA0304

   Plasmid

   #165612

   Purpose

   Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   NotI site replaced the CAN1 spacer in parent vect…

   Promoter

   SNR52

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGG426_UV5

   Plasmid

   #165608

   Purpose

   Vector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   lacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   D1135V, S1136R, D1332K and R1333G mutations in Sp…

   Promoter

   lacUV5 driving Cas9 VRKG and UV5 driving sgRNA

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cas9_nonPuro_OSS

   Plasmid

   #186652

   Purpose

   Cas9 plasmid to express the Cas9 in OSS cells

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Cas9 Expression Plasmid
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE

   Plasmid

   #140229

   Purpose

   Dox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFP

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   AntiCRISPR protein AcrVA1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ5079_pHR_PGK_sfGFP_CoV-F1

   Plasmid

   #155303

   Purpose

   SARS-CoV-2 fluorescent reporter 1

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   Superfolder GFP fused with SARS-CoV-F1 (ORF1ab Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

    Plasmid

    #107307

    Purpose

    Encoding an SPH activation system

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Insert

    CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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