We narrowed to 8,244 results for: 11
-
Plasmid#182279PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcAMPFIRE-L (cAMP sensor)
ExpressionMammalianAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_FOXA1_#2
Plasmid#70096PurposeExpression of shRNA to human FOXA1, puromycin selectionDepositorAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3_cAMPFIRE-H
Plasmid#182281PurposeMammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcAMPFIRE-H (cAMP sensor)
ExpressionMammalianAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9(D10A)
Plasmid#74495PurposeNicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10ADepositorInsertCas9 D10A
UseSynthetic BiologyExpressionBacterialMutationD10APromoterpTetoAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-NIR-GECO2G
Plasmid#159605PurposeAAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2GDepositorHas serviceAAV1, AAV2, and AAV9InsertNIR-GECO2G
UseAAVExpressionMammalianPromoterCAGAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSR660
Plasmid#65216PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-8 cap
UseAAVExpressionInsectPromoterP10 and polyhedrinAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tet-pLKO-FOXM1-shRNA-84
Plasmid#89496PurposeLentiviral vector with dox inducible expression of shRNA targeting human FOXM1.DepositorInsertFOXM1 (FOXM1 Human)
UseLentiviral and RNAi; Doxycycline inducibleExpressionMammalianPromoterH1/TOAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
STARR-seq luciferase validation vector_ORI_CMV
Plasmid#99312PurposeLuciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCMV enhancer
UseLuciferase; Starr-seq luciferase validation vectorExpressionMammalianAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAP114
Plasmid#120799PurposeE. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)DepositorInsertPxyl::mCherryOpt
ExpressionBacterialMutation1.4 kb xylR-Pxyl DNA fragment from C. difficile R…PromoterPxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUW-ubiquitin-EphB3-SV40-GFP (B3G)
Plasmid#65443PurposeExpression of EPHB3 and GFP in mammalian cellsDepositorAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-HA-Nr4a1
Plasmid#160941PurposeGeneration of retrovirus for the overexpression of Nr4a1DepositorAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQHA-USP11 CS puroR
Plasmid#46750PurposeRetroviral vector that expresses catalytically inactive form of HA-tagged human USP11DepositorInsertUSP11 (USP11 Human)
UseRetroviralTagsHAExpressionMammalianMutationC318S--catalytically inactivePromoterCMVAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-Frankenbody-FLAG-Halo
Plasmid#175296PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with HaloTag
TagsHaloTagExpressionMammalianAvailable sinceOct. 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PGL4.11-COL1A1mSMAD
Plasmid#230917PurposeTested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuman COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
UseLuciferaseTagsLuciferase-luc2pExpressionBacterial and MammalianMutationCOL1A1 promoter with mutant potential SMAD bindin…PromoterCOL1A1Available sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-4
Plasmid#172770PurposeBacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-4
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIA33
Plasmid#120803PurposeE. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfpDepositorInsertsdeactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein
UseCRISPRExpressionBacterialMutationBase-pairing region targets red fluorescent prote…PromoterPgdh and PxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRET_hNgn1_UBC_Bla
Plasmid#61473Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection markerDepositorInsertsUseLentiviralAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX
Plasmid#164611PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEndocannabinoid sensor GRAB_eCB2.0
ExpressionMammalianMutationS383TPromoterCMVAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1.Gαs-LgB113
Plasmid#134362PurposeNanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGαs-LgB113 (GNAS Human)
TagsHAExpressionBacterial and MammalianPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR646
Plasmid#65215PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-6 cap
UseAAVExpressionInsectPromoterP10 and polyhedrinAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only