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We narrowed to 10,966 results for: Vars;

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pETM33_ZYMND11_coiled-coil MYND

Plasmid

#178493

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

ZYMND11_coiled-coil MYND (ZMYND11 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalA (short) wt

Plasmid

#19723

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

NOTE: short splice variant Long Sp. Variant: MV…

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
MAC-GPR78

Plasmid

#158382

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

GPR78 (GPR78 Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP8

Plasmid

#158359

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP8 (ORF1ab )

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP10

Plasmid

#158361

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP10 (ORF1ab )

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC_C_EPHA8

Plasmid

#187761

Purpose

MAC-tagged gene expression of human EPHA8

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

EPHA8 (EPHA8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pRSETa-Amrose v3

Plasmid

#79539

Purpose

pRSETa (Invitrogen) plasmid expressing Amrose variant 3

Depositor

Randolph Caldwell

Article

Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

Insert

Amrose v3

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pETM33_SIN3A_PAH

Plasmid

#178486

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SIN3A_PAH (SIN3A Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX-MAPKAP1Del-FHH-IRES-Puro

Plasmid

#120706

Purpose

Expresses MAPKAP1 Truncation-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MAPKAP1 Isoform 1

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Deleted amino acids 193-522

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pNB784

Plasmid

#60163

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Use

Tags

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

SIC1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-HCF-1-D

Plasmid

#36997

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

HCF-1 shRNA (HCFC1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
PMKO-shNC

Plasmid

#64863

Purpose

Expresses a scrambled shRNA

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Insert

Scrambled shRNA

Use

Retroviral

Tags

Expression

Mutation

Promoter

u6

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER.retro.puro-HCF-1-B

Plasmid

#36996

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

HCF-1 shRNA (HCFC1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only
MAC_N_LHX4

Plasmid

#167778

Purpose

Expresses LHX4 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

LHX4 (LHX4 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR1A-3xFlag-S242R-MEFV

Plasmid

#167020

Purpose

Gateway entry plasmid with 3xFlag-pyrin (S242R/PAAND variant)

Depositor

Thomas Henry

Article

Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

Insert

MEFV (MEFV Human)

Use

Gateway entry vector

Tags

3xFlag

Expression

Mutation

serine 242 in arginine (S242R)

Promoter

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFA3

Plasmid

#46790

Purpose

To express ATPase inactive Fun30 in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 (FUN30 Budding Yeast)

Use

Tags

V5

Expression

Yeast

Mutation

ATP-binding site mutated replaced lysin 603 by ar…

Promoter

PGAL1

Available since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3 humanN-Flag SadBS

Plasmid

#66881

Purpose

Mammalian expression vector for expressing N-terminally Flag-tagged short isoform of SadB

Depositor

Maria Alvarado-Kristensson

Article

Eklund et al J Biol Chem. 2014 Aug 1;289(31):21360-73

Insert

SadB (BRSK1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pBW105

Plasmid

#111137

Purpose

For the expression of rat AANAT with Met-Leu-Ser N-terminus in S. cerevisiae

Depositor

Alexander Varshavsky

Article

Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

Insert

MLS-ratAANAT-3FLAG (Aanat Rat)

Use

Tags

3x Flag

Expression

Yeast

Mutation

Promoter

Gal1

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pmCellFree _KA2_ZIKV NS5

Plasmid

#145368

Purpose

Cell-free/mammalian expression vector for Zika virus NS5 protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS5

Use

Tags

8xHis and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-RHEB-IRES-Puro

Plasmid

#120576

Purpose

Expresses Flag-RHEB fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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