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pAc5.1B-lambdaN-HA-DmBam-M24E_AG

Plasmid

#148825

Purpose

Insect Expression of DmBam-M24E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam-M24E (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBam-V32E_AG

Plasmid

#148826

Purpose

Insect Expression of DmBam-V32E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam-V32E (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBam_AA

Plasmid

#148287

Purpose

Insect Expression of DmBam

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBam_AA

Plasmid

#148289

Purpose

Insect Expression of DmBam

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam (bam Fly)

Expression

Insect

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-C1-DmBam_AG

Plasmid

#148837

Purpose

Mammalian Expression of DmBam

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam (bam Fly)

Expression

Mammalian

Mutation

one non silent mutation A239 to S, described as …

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-DmBam_AG

Plasmid

#148840

Purpose

Mammalian Expression of DmBam

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam (bam Fly)

Expression

Mammalian

Mutation

one non silent mutation A239 to S, described as …

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pnYC-NvM-DmBam_141-442_AG

Plasmid

#148833

Purpose

Bacterial Expression of DmBam_141-422

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_141-422 (bam Fly)

Expression

Bacterial

Mutation

one non silent mutation A239 to S, described as …

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_MYL6_EF-hand

Plasmid

#109794

Purpose

Protein expression and purification of MYL6_EF-hand

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

MYL6_EF-hand (MYL6 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMT3-RhoGC D380E

Plasmid

#100035

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC D380E

Tags

1D4 (TETSQVAPA)

Expression

Mammalian

Mutation

D380E

Promoter

Adenovirus major late promoter

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMT3-RhoGC D380N

Plasmid

#100034

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC D380N

Tags

1D4 (TETSQVAPA)

Expression

Mammalian

Mutation

D380N

Promoter

Adenovirus major late promoter

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87 N184X

Plasmid

#92373

Purpose

Inducible expression of M87 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted, N184X

Promoter

Tet-responsive P tight

Available Since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP S245X with 5'UTR

Plasmid

#92376

Purpose

Inducible expression of S245X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

S245X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87 S245X

Plasmid

#92374

Purpose

Inducible expression of M87 S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted, S245X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1 S245X

Plasmid

#92372

Purpose

Inducible expression of M1S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, S245X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1 N184X

Plasmid

#92371

Purpose

Inducible expression of M1 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, N184X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/C448Y with 5'UTR

Plasmid

#89318

Purpose

expression of C448Y spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87C448Y

Plasmid

#89329

Purpose

Inducible expression of M87 C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted, C448Y

Promoter

Tet-responsive P tight

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP C448Y with 5'UTR

Plasmid

#89325

Purpose

Inducible expression of C448Y spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

C448Y

Promoter

Tet-responsive P tight

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1C448Y

Plasmid

#89326

Purpose

Inducible expression of M1C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, C448Y

Promoter

Tet-responsive P tight

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mRuby2

Plasmid

#40260

Purpose

RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Insert

mRuby2

Tags

His6 and Xpress

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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