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pET28_human-pro-IL-18_LES33AAA

Plasmid

#214326

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

LES33AAA

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_DDEN29ENGD

Plasmid

#214325

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

DDEN29ENGD

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_V47N_I48N

Plasmid

#214324

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

V47N_I48N

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGA_pET28

Plasmid

#167326

Purpose

Expresses TFAM HMGA mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA domain (43-122)

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pScSR beta deltaN Flag

Plasmid

#13729

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRP102 Budding Yeast)

Use

Tags

6xHis and Flag

Expression

Bacterial

Mutation

expresses residues 31-244 of S. cerevisiae SRβ wi…

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_DeltaN24

Plasmid

#214327

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Deletion of first 24 amino acids

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

Plasmid

#89508

Purpose

Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

Insert

rAPOBEC1-nCas9 (D10A)-UGI-NLS

Use

Tags

UGI and rAPOBEC-XTEN

Expression

Bacterial

Mutation

D10A mutation of Cas9

Promoter

T7

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-HISx6-SIMx6

Plasmid

#116938

Purpose

Bacterial expression vector for 6 sim

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

6 sim

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-HISx6-SUMOx10

Plasmid

#116937

Purpose

Bacterial expression vector for 10 sumo

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

10 SUMO

Use

Tags

HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET28_Human_SAT1_Cterm_His

Plasmid

#220487

Purpose

heterologous expression of SSAT1 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Human_SAT1_Nterm_His

Plasmid

#220482

Purpose

heterologous expression of SSAT1 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-Mff(1-61)-PP-GST

Plasmid

#73042

Purpose

Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site

Depositor

David Chan

Article

Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

Insert

Mff(1-61) (Mff Mouse)

Use

Tags

GST-tag and PreScission protease site

Expression

Bacterial

Mutation

Truncation containing amino acids 1-61

Promoter

T7

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_dHMGA_pET28

Plasmid

#167329

Purpose

Expresses TFAM dHMGA mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing linker, HMGB, and C-…

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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H6SUMO-IntC-P53(304-393)

Plasmid

#200316

Purpose

bacterial expression of P53 TET-CTD for segmental labeling

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (304-393) (TP53 Human)

Use

Tags

His6-SUMO and designed intein C

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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YFP-FKBPx5-His

Plasmid

#103785

Purpose

expresses His-tagged iPOLYMER component in E. coli for protein purification

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

YFP-FKBPx5-His

Use

Tags

EYFP, His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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HOXB2 (human) HIS-tag pET

Plasmid

#8522

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB2 (HOXB2 Human)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Starts at AA #2; makes protein by Western

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pET28-p53 (1-73) (72P)

Plasmid

#62083

Purpose

Expression of human p53 (1-73) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Use

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72P

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
p28atDarpp

Plasmid

#108318

Purpose

Expresses tDarpp in E.coli

Depositor

Jamil Momand

Article

Momand et al FEBS Open Bio. 2017 Aug 29;7(9):1328-133

Insert

tDarpp (PPP1R1B Human)

Use

Tags

6 Histadine

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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HOXB1 (human) HIS-tag pET

Plasmid

#8520

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB1 (HOXB1 Human)

Use

Tags

His and T7

Expression

Bacterial

Mutation

PCR from cDNA clone

Promoter

Available since

July 6, 2006

Availability

Academic Institutions and Nonprofits only
pET28-p53 (1-73) (72R)

Plasmid

#62082

Purpose

Expression of human p53 (1-73) (72R) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Use

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72R

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only