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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,332 results for: pET28a

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Showing: 1201 - 1220 of 1332 results

  3. hCD12

    Plasmid
    #225541
    Purpose
    Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.
    Depositor
    David Christianson
    Article
    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi
    Insert
    Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    225541-plasmid-map-sequence-id-446604

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  7. pET28-AviTag-DogTag-MBP

    Plasmid
    #171773
    Purpose
    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogTag-MBP
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171773-plasmid-map-sequence-id-338517

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  9. SpyTag003-OspC

    Plasmid
    #159998
    Purpose
    Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells
    Depositor
    Mark Howarth
    Article
    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:
    Insert
    SpyTag003-OspC (20-209) C129S
    Tags
    His6-tag and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    C129S mutation improves expression level.
    Promoter
    T7
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159998-plasmid-map-sequence-id-316165

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Showing: 1201 - 1220 of 1332 results