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Plasmid#87936PurposeExpression in S.cerevisiae of FjTALDepositorInsertFjTAL
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP-TAG
Plasmid#158131PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)DepositorInsertsfGFP
UseTagsExpressionYeastMutationIntroduced a stop codon in place of the tyrosine …PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCT
Plasmid#60620PurposePlasmid encoding iCas9 and tracrRNA on pRS415 backboneDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y98N-pBabe-puro
Plasmid#19236DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tyrosin 98 to AsparaginPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDEST51-LRRK2-Y1699C
Plasmid#25084DepositorInsertLRRK2 (LRRK2 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationTyrosine 1699 mutated to CysteinePromoterAvailable sinceJuly 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y112N-pBabe-puro
Plasmid#19238DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tyrosin 112 to AsparaginPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y112H-pBabe-puro
Plasmid#19237DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tyrosin 112 to HistidinPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCR-Blunt II-TOPO-PTPRD
Plasmid#29705DepositorInsertProtein tyrosine phosphatase receptor-type delta (PTPRD Human)
UseCloning vectorTagsExpressionMutationnonePromoterAvailable sinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2
Plasmid#111269Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y98H-pBabe-puro
Plasmid#19235DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tyrosin 98 to HistidinPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
actc1b-BTK-mKate2
Plasmid#109501PurposeMuscle specific zebrafish expression of mKate2 fused to the PH domain from Human Bruton tyrosine kinase (BTK). Binds PtdIns(3,4,5)P3.DepositorInsertactc1b-BTK-mKate2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 FYN
Plasmid#67106PurposeStandard for cell-free expression benchmarking.DepositorInsertFYN (FYN Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-PTPRD-G1707R
Plasmid#32526DepositorInsertProtein tyrosine phosphatase receptor-type delta G1707R (PTPRD Human)
UseLentiviralTagsExpressionMammalianMutationG1707RPromoterAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-PTPRD-P1690F
Plasmid#32525DepositorInsertProtein tyrosine phosphatase receptor-type delta P1690F (PTPRD Human)
UseLentiviralTagsExpressionMammalianMutationP1690FPromoterAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ36-72
Plasmid#232060PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72DepositorInsertNeurofilament-Heavy tail domain lacking residues 36-72
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ462-638
Plasmid#232061PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638DepositorInsertNeurofilament-Heavy tail domain lacking residues 462-638
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMtail
Plasmid#232056PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domainDepositorInsertNeurofilament-Medium tail domain
UseTags6xHis, thrombinExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHtail
Plasmid#232057PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domainDepositorInsertNeurofilament-Heavy tail domain
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAMC042z21
Plasmid#209747PurposeRepA(N99D) SC101 T0-BS7 proD SD8 sfGFP( V93AGGA Y151UAGA) ProK 5 tRNA operon tetA-orfL blaDepositorInsertsfGFP - tRNA operon
UseTagsExpressionBacterialMutationsfGFP - Valine 93 AGGA quadruplet codon, Tyrosine…PromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y115S/Y132S/Y139S
Plasmid#216408PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutationsDepositorInsertEfg1-ΔDBD-Y115S/Y132S/Y139S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 115, 132 and 139 to Serine, and …PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only