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NET30 pEGFP-N2 (815)

Plasmid

#62011

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET30 (MOSPD3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
NET55 pEGFP-N2 (610)

Plasmid

#61992

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET55 (APH1B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pOPINK-TRABID (NZF1-3, aa 1-178)

Plasmid

#66185

Purpose

Expresses TRABID NZF1-3 (aa 1-178) in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

TRABID (ZRANB1 Human)

Use

Tags

His6-GST

Expression

Bacterial

Mutation

only codes for amino acids 1-178 of TRABID

Promoter

T7

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
GFP-rab11 WT

Plasmid

#12674

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-rab7 WT

Plasmid

#12605

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
DsRed-rab7 DN

Plasmid

#12662

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Use

Tags

DsRed and HA

Expression

Mammalian

Mutation

T22N

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-rab7 DN

Plasmid

#12660

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

T22N

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
DsRed-rab11 WT

Plasmid

#12679

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Use

Tags

DsRed and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
DsRed-rab7 WT

Plasmid

#12661

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Use

Tags

DsRed and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
LBR pEGFP-N2 (646)

Plasmid

#61996

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

LBR (LBR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINK-TRABID (NZF1, aa 1-33)

Plasmid

#65437

Purpose

Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

TRABID (ZRANB1 Human)

Use

Tags

His6-GST

Expression

Bacterial

Mutation

only codes for amino acids 1-33 of TRABID, which …

Promoter

T7

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
Sec61 beta pAcGFP-C1 (802)

Plasmid

#62008

Purpose

mammalian expression of GFP-Sec61 beta

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

Sec61 beta (SEC61B Human)

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pIS1-Vim/Actb5UTR-renilla

Plasmid

#38238

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Vim promoter with Actb 5UTR/Renilla (Vim, Actb Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Actb promoter (1000 nt upstream) and 5' UTR …

Promoter

Endogenous Vim

Available since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pBV-Luc/Del-6

Plasmid

#14969

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-109/+334) (MYC Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-109/+334 (relative to the P2 transcription initi…

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
NET37 pEGFP-N2 (607)

Plasmid

#61990

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET37 (KIAA1161 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pBV-Luc/Frag-F

Plasmid

#14053

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-109/-3) (MYC Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-109/-3 (relative to the P2 transcription initiat…

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
DsRed-rab11 DN

Plasmid

#12680

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Use

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-rab11 DN

Plasmid

#12678

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-Flag-Rheb-4Rs

Plasmid

#165022

Purpose

expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)

Depositor

Ken Inoki

Article

Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

Insert

Rheb-4Rs (RHEB Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

change lysine residues on position 109, 135, 151,…

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneoMyc UNC119B

Plasmid

#128333

Purpose

Mammalian expression vector of Myc-tagged human UNC119B

Depositor

Yutaka Hata

Article

Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

Insert

UNC119B (UNC119B Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge