We narrowed to 30,669 results for: des.2

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pGTag Hygro EGFP nLAP +2

Plasmid

#194307

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-MSulf-2

Plasmid

#13008

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

mouse sulfatase 2 (Sulf2 Mouse)

Tags

6xHis and Myc

Expression

Mammalian

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 Spike-d18

Plasmid

#164583

Purpose

A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

Depositor

Jason Sheltzer

Insert

SARS-CoV-2 Spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

Deleted amino acids 1256-1273

Promoter

EF1a

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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PLKO.1-SMG6-CDS-2

Plasmid

#136047

Purpose

SMG6 shRNA (Targeting CDS #2) inserted into the PLKO.1 plasmid (AAGGAGTTCCAGGTGTTACTG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

SMG6 (SMG6 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagD shRNA #2

Plasmid

#30322

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagD shRNA (RRAGD Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 2, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-ef1a-DIO-eGCaMP(2+)

Plasmid

#201150

Purpose

AAV virus production for Cre recombinase dependent expression of eGCaMP(2+)

Depositor

Andre Berndt

Article

Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

Insert

eGCaMP_2+

Use

AAV, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagB shRNA #2

Plasmid

#26628

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagB shRNA (RRAGB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-2 SNAP-Syk tSH2

Plasmid

#113028

Purpose

for purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

SNAP-Syk tSH2

Tags

SNAP

Expression

Bacterial

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pEBB HA BIR 2-3

Plasmid

#25691

Depositor

Colin Duckett

Article

Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

Insert

XIAP (XIAP Human)

Tags

HA

Expression

Mammalian

Mutation

amino acid 124RDH-LEE350 (BIR 2&3)

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-shNup133-2

Plasmid

#87333

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-nMyc-m2_3'UTR

Plasmid

#31860

Depositor

Robert Blelloch

Article

Melton et al Nature. 2010 Feb 4;463(7281):621-6. Epub

Insert

nMyc mutant-1 3'UTR

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Second Let-7c Seed changed from TACCTC to TAACGC

Promoter

SV40

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pdFLPtag-SA-SD-2-GFSTF

Plasmid

#247951

Purpose

Conditional tagging of endogenous proteins with GFSTF in open reading frame 2, with dual membrane labelling

Depositor

Sha Liu

Article

Li et al Sci Rep. 2025 Nov 27;15(1):45542. doi: 1

Insert

GFSTF

Tags

GFSTF

Expression

Insect

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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p8390 LentiCRISPRv2 Neo sgNT-2

Plasmid

#221650

Purpose

Expression of non-targeting control sgRNA

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

non-targeting sgNT-2

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro BFP nLAP -2

Plasmid

#194325

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro BFP nLAP +2

Plasmid

#194319

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pVH230-2-Tier1-PmPGK1-YPet

Plasmid

#169521

Purpose

Tier-1 vector encoding PmPGK1-driven YPet expression (PmPGK1-YPet-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven yellow fluorescent protein Ypet

Expression

Mammalian

Promoter

PPGK

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pVH237-2-Tier1-PmPGK1-TtgA

Plasmid

#169584

Purpose

Tier-1 vector encoding PmPGK1-driven TtgA expression (PmPGK1-TtgA-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

Regulatory protein TtgR fused to a VP16 domain

Expression

Mammalian

Promoter

PPGK

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pPD187 Tri-AND Gate, 2 repeats

Plasmid

#138940

Purpose

EYFP reporter vector for the Logic 37 AND 43 AND 158 2 repeats promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

Logic 37 AND 43 AND 158 2 repeats

Use

Synthetic Biology

Expression

Mammalian

Promoter

Logic 37 AND 43 AND 158 2 repeats

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-nMyc-m1_3'UTR

Plasmid

#31859

Depositor

Robert Blelloch

Article

Melton et al Nature. 2010 Feb 4;463(7281):621-6. Epub

Insert

nMyc mutant-1 3'UTR

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

first Let-7c Seed changed from TACCTC to TAACGC

Promoter

SV40

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pBbS5a-Opto-Cre-Vvd-2

Plasmid

#160400

Purpose

Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

Depositor

Mary Dunlop

Article

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

Opto-Cre-Vvd-2

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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