2,226 results
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Plasmid#206106Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit with a single Bungarotoxin Binding Site motif inserted after aa549HADepositorInsertcacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 R57A, R59A pMT2
Plasmid#206112Purposeexpression of rat Cav2.1 calcium channel with R57A and R59A mutations that prevent G-protein modulationDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationR57A, R59APromoterAd MLP/TPL/SV40Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
rUBR5_2383-2442_pGEX6P1
Plasmid#209791PurposeBacterial expression of UBR5 Mlle domainDepositorInsertMlle domain of UBR5 (Ubr5 Rat)
UseTagsGSTExpressionBacterialMutationaa 2383-2442 onlyPromotertacAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSyt1C2AB(80-421)msGFP
Plasmid#209009PurposeRecombinant protein expression of truncated syt1 tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis and msfGFPExpressionBacterialMutationaa 80-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(96-421)
Plasmid#209006PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 96-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETmsGFPSyt1(80-142)
Plasmid#209012PurposeRecombinant protein expression of truncated syt1 tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-msfGFPExpressionBacterialMutationaa 80-142 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETmsGFPSyt1(80 JuxtaK-142)
Plasmid#209013PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-msfGFPExpressionBacterialMutationaa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…PromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSyt1C2AB(143-421)msGFP
Plasmid#209010PurposeRecombinant protein expression of truncated syt1 tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis and msfGFPExpressionBacterialMutationaa 143-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(80 JuxtaK-421)
Plasmid#209008PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutationsDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…PromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynWSyt1C2AB(80 JuxtaK-421)msGFP
Plasmid#209016PurposeOverexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cellsDepositorInsertsyt1 (Syt1 Rat)
UseLentiviralTagsmsfGFPExpressionMammalianMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…PromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(143-421)
Plasmid#209007PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 143-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSyt1C2AB(80 JuxtaK-421)msGFP
Plasmid#209011PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis and msfGFPExpressionBacterialMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…PromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynWSyt1C2AB(143-421)msGFP
Plasmid#209015PurposeOverexpression of truncated syt1 tagged with GFP in mammalian cellsDepositorInsertsyt1 (Syt1 Rat)
UseLentiviralTagsmsfGFPExpressionMammalianMutationaa 143-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
cacnb1b pCAGGS
Plasmid#206102Purposeexpression of rat beta1b calcium channel auxillary subunitDepositorInsertcacnb1b (Cacnb1 Rat)
UseTagsExpressionMammalianMutationPromoterCMV/B-actinAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
An R57A, R59A HA CAAX pMT2
Plasmid#206126PurposeCav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif and a HA tagDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationR57A, R59APromoterAd MLP/TPL/SV40Available sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
An CAAX pMT2
Plasmid#206118Purposeexpression of Cav2.1 N-terminus (amino acids 1-100) with C-terminal CAAX (last 10 aa of H.Ras) motifDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationPromoterAd MLP/TPL/SV40Available sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-RnCENP-O-GFP
Plasmid#205179PurposeExpresses rat CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorInsertCENP-O (Cenpo Rat)
UseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationWild type rat CENP-OPromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-RnCENP-O-11NS-GFP
Plasmid#205181PurposeExpresses rat CENP-O with 11 mouse-specific point mutations in residues under positive selection; C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorUseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationRat CENP-O with 11 mouse-specific point mutations…PromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-RnCENP-O-80-GFP
Plasmid#205182PurposeExpresses chimeric rat CENP-O with mouse CENP-O N-terminus; C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorUseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationChimeric rat CENP-O with mouse CENP-O N terminal …PromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-RnCENP-O-203-GFP
Plasmid#205183PurposeExpresses chimeric rat CENP-O with mouse CENP-O middle region including central RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorUseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationChimeric rat CENP-O with mouse CENP-O middle regi…PromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-RnCENP-O-298-GFP
Plasmid#205184PurposeExpresses chimeric rat CENP-O with mouse CENP-O C-terminal RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorUseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationChimeric rat CENP-O with mouse CENP-O C-terminal …PromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-MmCENP-O-R298-GFP
Plasmid#205185PurposeExpresses chimeric mouse CENP-O with rat CENP-O C-terminal RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorUseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationChimeric mouse CENP-O with rat CENP-O C-terminal …PromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-RnCENP-O-298-5NS-GFP
Plasmid#205742PurposeExpresses chimeric rat CENP-O with mouse CENP-O C-terminal RWD domain and 5 mouse-specific point mutations; C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorUseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationChimeric rat CENP-O with mouse CENP-O C-terminal …PromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMyosin-miRFP704nano
Plasmid#206194PurposeLC-Myosin labelled with near-infrared fluorescent protein miRFP704nanoDepositorInsertmiRFP704nano-LC-Myosin
UseTagsmiRFP704nanoExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLAMP1-miRFP704nano
Plasmid#206195PurposeLAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP704nanoDepositorInsertLAMP1-miRFP704nano
UseTagsmiRFP704nanoExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW pHluorin-SYT9 IRES mRuby3
Plasmid#195698PurposeLentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoterDepositorInsertSyt9 (Syt5 Rat)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2A
Plasmid#195710PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2A domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 96-264 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2B
Plasmid#195711PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2B domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 248-421 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT1 C2AB
Plasmid#195712PurposeBacterial expression plasmid encoding GST-tagged SYT1 C2AB domainDepositorInsertSyt1 (Syt1 Rat)
UseTagsGSTExpressionBacterialMutationencodes amino acids 96-421 of mouse SYT1PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET (SUMO)-Syt9
Plasmid#195713PurposeBacterial expression plasmid encoding SUMO-tagged full-length Syt9DepositorInsertSyt9 (Syt5 Rat)
UseTagsGST-SUMOExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only