We narrowed to 21,797 results for: Sis
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Plasmid#138359PurposeGateway compatible middle entry clone contains human full length NFR2F2 gene with stop codonDepositorInsertHuman NR2F2
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSAR-Cre-F2A-I-SceI
Plasmid#62302PurposeAnopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed markerDepositorInsertCre recombinase, I-SceI nuclease
UseTagsExpressionInsectMutationPromotervasaAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2-sgControl
Plasmid#230080PurposeCrispr knock out controlDepositorInsertnon-specific sequence control
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsA
Plasmid#107558PurposePlasmid for titrating synthesis of FtsA with doxycyclineDepositorInsertftsA
UseSynthetic BiologyTagsExpressionMutationPromoterpLtetOAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pV1F
Plasmid#205084PurposeProkaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.DepositorInsertAtVIP1
UseTagsExpressionBacterialMutationPromoteragorbacterium virB operon promoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGE1108
Plasmid#202772PurposeCRISPR-KO in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsZ
Plasmid#89656PurposePlasmid for titrating synthesis of FtsZ with doxycyclineDepositorInsertGTP-binding tubulin-like cell division protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM126
Plasmid#105134PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-DERA
Plasmid#203225PurposeFor protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertdeoxyribose aldolase
UseTagsN-terminal TEV cleavable 6xHis tagExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Aurli_150841_GZG
Plasmid#162563PurposeInactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.DepositorInsertshble
UseUnspecifiedTagsExpressionMutationPromoterGAPDHAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-ClpX
Plasmid#89657PurposePlasmid for titrating synthesis of ClpX with doxycyclineDepositorInsertclpX
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4-vas2-integrase-R3
Plasmid#62299PurposephageC31 integrase-expressing helper plasmid for Anopheles transgenesis, vasa promoterDepositorInsertphageC31 integrase under control of vasa promoter and terminator
UseTagsHA and SV40 NLSExpressionInsectMutationPromotervasaAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTX1-SMN1-exon7-ESE1
Plasmid#126041PurposeIn-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysisDepositorInsertSMN1 exon7 ESE1
UseTagsExpressionMutationPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUB-cHyPer2
Plasmid#84728PurposeExpresses HyPer2 in plant cells-biosensor for Hydrogen peroxideDepositorInsertHyPer2
UseTagsExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMARO
Plasmid#178086PurposeDerivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.DepositorInsertTcR (tetA/tetR)
UseTagsExpressionBacterialMutationTetracycline resistance cassette is flanked by Ec…PromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2xVlk1-3xFlag
Plasmid#172773PurposemRNA synthesis for injection into embryosDepositorInsertVlk1 (pkdcc.1.S Frog)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSPA1L
Plasmid#177660PurposeRecombinant protein synthesis of human HSPA1LDepositorInsertHSPA1L (HSPA1L Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-scFv-p65-HSF1-Blast
Plasmid#192652Purpose3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance markerDepositorInsertscFv-p65-HSF1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDE43-MCK
Plasmid#49523PurposeKanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCORE-Kp53
Plasmid#72241PurposeContains the CORE cassette with KanMX4 and human p53 gene markersDepositorInsertsUseTagsExpressionBacterialMutationV122A mutationPromoterAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pSBY3_ligD-HygR
Plasmid#104625PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.DepositorInsertFnCpf1
UseCRISPRTagsExpressionBacterialMutationFnCpf1 codon-optimized C.glutamicumPromoterhsp60Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWJB30
Plasmid#126505PurposeSalmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30DepositorInsertadrA-8xHis
UseTags8xHisExpressionBacterialMutationPromoterPBAD30Available sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUB-cSypHer
Plasmid#84733PurposeExpresses SypHer in plant cells-biosensor for pHDepositorInsertSypHer
UseTagsExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-flag-mKdm4d(H189A)-polyA
Plasmid#61554PurposeFor mRNA synthesis or expression in mammalian cellsDepositorInsertKdm4d (catalytic mutant)
UseTagsflagExpressionMammalianMutationchanged Histidine 189 (of Kdm4d) to AlaninePromoterCMV and T7Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETCON-TNF
Plasmid#67748PurposeTumor Necrosis Factor Alpha Yeast Display PlasmidDepositorInsertTNF-Alpha
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRecLV103-GFP-PGBD5
Plasmid#65409PurposeLentiviral expression vector for fusion of GFP with human PGBD5DepositorInsertPGBD5 (PGBD5 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGE496
Plasmid#153248PurposeM6 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE495
Plasmid#153247PurposeM5 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCORE-Hp53
Plasmid#72242PurposeContains the CORE cassette with hyg and human p53 gene markersDepositorInsertsUseTagsExpressionBacterialMutationV122A mutationPromoterAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM2937
Plasmid#84112Purposetarget miRNA2937 for destruction in Arabidopsis thalianaDepositorInsertSTTM2937
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE336
Plasmid#153245PurposeM4 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE341
Plasmid#153250PurposeM7 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM159
Plasmid#84084Purposetarget miRNA159 for destruction in Arabidopsis thalianaDepositorInsertSTTM159
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941DR5-PacI-ath-STTM390
Plasmid#84117Purposetarget miRNA390 for destruction in Arabidopsis thalianaDepositorInsertSTTM390
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM858a/b
Plasmid#84177Purposetarget miRNA858 for destruction in Arabidopsis thalianaDepositorInsertSTTM858a/b
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM156/157
Plasmid#84083Purposetarget miRNA156/157 for destruction in Arabidopsis thalianaDepositorInsertSTTM156/157
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM828
Plasmid#84157Purposetarget miRNA828 for destruction in Arabidopsis thalianaDepositorInsertSTTM828
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCSh3-mRFP
Plasmid#170983PurposeEncodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.DepositorInsertmRFP
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM843
Plasmid#84169Purposetarget miRNA843 for destruction in Arabidopsis thalianaDepositorInsertSTTM843
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM845
Plasmid#84170Purposetarget miRNA845 for destruction in Arabidopsis thalianaDepositorInsertSTTM845
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-DGK1-3HA
Plasmid#198595PurposeDGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.DepositorInsertDGK1 (DGK1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-PAH1-3HA
Plasmid#198597PurposePAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.DepositorInsertPAH1 (PAH1 Budding Yeast)
UseTags3HAExpressionYeastMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXSE901B
Plasmid#91713PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistanceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM173
Plasmid#84101Purposetarget miRNA173 for destruction in Arabidopsis thalianaDepositorInsertSTTM173
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM168
Plasmid#84093Purposetarget miRNA168 for destruction in Arabidopsis thalianaDepositorInsertSTTM168
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM160
Plasmid#84085Purposetarget miRNA160 for destruction in Arabidopsis thalianaDepositorInsertSTTM160
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
SH4-FRB-mRFP
Plasmid#100741PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorInsertFyn
UseTagsFRB and mRFPExpressionMammalianMutationSH4 domain onlyPromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM5026
Plasmid#84192Purposetarget miRNA5026 for destruction in Arabidopsis thalianaDepositorInsertSTTM5026
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only