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We narrowed to 1,293 results for: Ncl

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Showing: 1221 - 1240 of 1293 results

  2. mEos3.2GG

    Plasmid
    #203756
    Purpose
    Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    KRas c-term (KRAS Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    203756-plasmid-map-sequence-id-404785

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  3. MKC62_HBB(HBA1reg-HBA1-2A-YFP)

    Plasmid
    #232408
    Purpose
    AAV production plasmid for HBA1-2A-YFP vector from Fig. 2 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank full HBA1-2A-YFP cassette.
    Depositor
    Kyle Cromer
    Article
    Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0
    Insert
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Expression
    Mammalian
    Available Since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232408-plasmid-map-sequence-id-466143

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  4. pDmelOR

    Plasmid
    #126476
    Purpose
    Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    DmelOrco (Orco Fly)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens; includes a rec…
    Promoter
    CMV
    Available Since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126476-plasmid-map-sequence-id-246523

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  7. lentiMutB3GALT5catdead-blast

    Plasmid
    #220869
    Purpose
    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Article
    Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    encodes D156A and D243A mutations; also, nucleoti…
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220869-plasmid-map-sequence-id-440865

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  10. PET.SUMO eIF4G1 +MIC 1-200

    Plasmid
    #158792
    Purpose
    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1 (with microexon) codon optimized
    Tags
    6xHIS and SUMO
    Expression
    Bacterial
    Mutation
    amino acids 1-200
    Promoter
    T7
    Available Since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158792-plasmid-map-sequence-id-309816

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  11. YFP(1-158)-gamma-2 in pcDNAI/Amp

    Plasmid
    #55762
    Purpose
    An amino-terminal YFP fragment was fused to Ggamma2. When co-expressed with a carboxyl terminal YFP or CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    YFP(1-158)-gamma-2 (GNG2 Aequorea victoria, Human)
    Tags
    YFP(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55762-plasmid-map-sequence-id-115200

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  14. pEF6_DmrB-[dltCARD]mCasp1(E102D103TRB_E121D122AA) (KS655)

    Plasmid
    #254936
    Purpose
    Murine Casp1 with its CARD replaced by DmrB, the 1st CDL cleavage site (E102D103) replaced by a Thrombin site, and the 2nd CDL cleavage site uncleavable (E121D122AA), in pEF6.
    Depositor
    Kate Schroder
    Article
    Boucher et al J Exp Med. 2018 Mar 5;215(3):827-840. do
    Insert
    Casp1 (Casp1 Mouse)
    Tags
    DmrB
    Expression
    Bacterial and Mammalian
    Mutation
    E102D103TRB_E121D122AA
    Promoter
    EF-1a, T7
    Available Since
    June 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    254936-plasmid-map-sequence-id-512354

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  19. pKlab1157-HBEGF-2a-3xFLAG-BRCA1(full-length)-P1749S-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248997
    Purpose
    This plasmid expresses the BRCA1 sequence with P1749S mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    P1749S
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248997-plasmid-map-sequence-id-495753

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  20. pKlab1158-HBEGF-2a-3xFLAG-BRCA1(full-length)-R1699Q-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248998
    Purpose
    This plasmid expresses the BRCA1 sequence with R1699Q mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    R1699Q
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248998-plasmid-map-sequence-id-495728

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Showing: 1221 - 1240 of 1293 results