We narrowed to 2,521 results for: crispr system

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pET28-T7-NlaCas3-NLS-6xHis

Plasmid

#180214

Purpose

Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas3c

Tags

6xHis and NLS

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPN460

Plasmid

#137878

Purpose

PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRa;mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

POGZ (POGZ Human)

Use

CRISPR

Expression

Mammalian

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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CSAC-Crys

Plasmid

#164967

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2

Use

AAV

Tags

mCherry

Promoter

hU6, hSyn

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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CSAC-Bmal1

Plasmid

#168296

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgBmal1-1-hU6-sgBmal1-3

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

Plasmid

#176273

Purpose

Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (sgTet) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-nCas9(D10A)-PmCDA1

Plasmid

#79617

Purpose

Expresses nCas9(D10A)-PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

PmCDA1

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-NRCH-PE2-BSD

Plasmid

#191101

Purpose

Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

NRCH-PE2-BSD

Use

CRISPR and Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPV-H1-ccdB-mEF1α-RiH

Plasmid

#100598

Purpose

piggyBac vector for sgRNA cloning

Depositor

Akitsu Hotta

Article

Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

Type

Empty backbone

Use

Piggybac vector

Expression

Mammalian

Promoter

Human H1 PolIII promoter, human FTH1 promoter, mo…

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pGTag-P2A-QFGal4-SV40pA

Plasmid

#155127

Purpose

Donor for precise CRISPR directed genomic integration of QFGal4 using the GeneWeld method

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

P2A-QFGal4

Use

CRISPR and Synthetic Biology

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHS1398

Plasmid

#205971

Purpose

MWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH crRNA

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1182

Plasmid

#205964

Purpose

NZ_CP025815 DinG HNH crRNA expression in pCOLADuet-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH crRNA

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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SL2-sgTelo-MTSa/BFP/pdCas9-C1

Plasmid

#162760

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting telomeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgTelo-MTSa)

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCK389.AAV

Plasmid

#177327

Purpose

aTc inducible MCP-SoxS double mutant expression for CRISPRa in E.coli. Contains constitutive dCas9 and scRNA expression

Depositor

James Carothers

Article

Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

Inserts

SoxS
dCas9

Use

CRISPR

Tags

MCP (MS2 Coat Protein)

Mutation

R93A,S101A

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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SL2-sgSat-MTSb/BFP/pdCas9-C1

Plasmid

#162761

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting centromeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgSat-MTSb)

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pZ004 (LseC2c2 locus)

Plasmid

#70170

Purpose

Expresses LseC2c2 locus with one spacer in array

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

LseC2c2 locus with one spacer in array

Expression

Bacterial

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only
pPN461

Plasmid

#137877

Purpose

PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

POGZ (POGZ Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pX552 EF1a DIO Chronos GFP_Vgat gRNA

Plasmid

#215277

Purpose

Expresses Vgat gRNA in a Cre dependent Chronos GFP vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Vgat gRNA

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pX552 EF1a DIO Chronos GFP_VgatTTT gRNA

Plasmid

#215278

Purpose

Expresses Vgat Control gRNA in a Cre dependent Chronos GFP vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Vgat control gRNA

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pX552-EF1a-DIO-mCherry_Dicer gRNA

Plasmid

#215279

Purpose

Expresses Dicer gRNA in a Cre dependent mcherry vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Dicer control gRNA

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pX552-hSyn-DIO-GCaMP8f_Grin1 gRNA

Plasmid

#215281

Purpose

Expresses Grin 1 gRNA in a Cre dependent GCaMP8f vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Grin1 gRNA

Use

AAV

Expression

Mammalian

Mutation

See Depositor Comments Below

Promoter

U6

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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