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SNX6-PX (27-171)

Plasmid

#119087

Purpose

Bacterial expression of human phox homology (PX) domain, SNX6-PX (27-171)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX6-PX (29-170) (SNX6 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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SH3PXD2A-PX (1-126)

Plasmid

#119115

Purpose

Bacterial expression of human phox homology (PX) domain, SH3PXD2A-PX (1-126)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SH3PXD2A-PX (1-126) (SH3PXD2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

May 25, 2024

Availability

Academic Institutions and Nonprofits only

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pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)

Plasmid

#181787

Purpose

Expresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ShTnsB, ShTnsC, ShCas12k-ShTniQ

Use

Tags

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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DJ-1 TEV site pET15b

Plasmid

#60687

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

TEV site was inserted (see comments below).

Promoter

T7

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
SNX12-PX (1-162)

Plasmid

#119093

Purpose

Bacterial expression of human phox homology (PX) domain, SNX12-PX (1-162)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX12-PX (1-162) (SNX12 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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SNX11-PX (7-167)

Plasmid

#119092

Purpose

Bacterial expression of human phox homology (PX) domain, SNX11-PX (7-167)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX11-PX (7-167) (SNX11 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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CFF-EGFP

Plasmid

#86061

Purpose

A mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Fibrocystin (Pkhd1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains luminal and transmembrane domains of CD8…

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pp32ΔCT

Plasmid

#67241

Purpose

pp32(1-149) cloned into pProEX HTb

Depositor

Cynthia Wolberger

Article

Huyton et al Protein Sci. 2007 Jul;16(7):1308-15. Epu

Insert

pp32 (ANP32A Human)

Use

Tags

His

Expression

Bacterial

Mutation

contains amino acid residues 1-149

Promoter

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
SNX3-PX (1-162)

Plasmid

#119084

Purpose

Bacterial expression of human phox homology (PX) domain, SNX3-PX (1-162)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX3-PX (1-162) (SNX3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA17_Sga-NLS(SV40) (KAC92)

Plasmid

#134334

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Sga with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA17_Sga

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA18_Sma-NLS(SV40) (KAC95)

Plasmid

#134335

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sma with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA18_Sma

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA19_Ssim-NLS(SV40) (KAC97)

Plasmid

#134336

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Ssim with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA19_Ssim

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA18_Sga-NLS(SV40) (KAC214)

Plasmid

#134338

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sga with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA18_Sga

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-AcrIIA19_Spse-NLS(SV40) (KAC215)

Plasmid

#134339

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Spse with C-terminal NLS (SV40)

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA19_Spse

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SGK3-PX (1-126)

Plasmid

#119117

Purpose

Bacterial expression of human phox homology (PX) domain, SGK3-PX (1-126)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SGK3-PX (1-126) (SGK3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-delta133HtrA2 S400D

Plasmid

#16156

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

S400D

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET20b-delta133HtrA2 S142A

Plasmid

#16153

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Sep 30. ():.

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

S142A

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
SNX31-PX (1-106)

Plasmid

#119110

Purpose

Bacterial expression of human phox homology (PX) domain, SNX31-PX (1-106)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX31-PX (1-106) (SNX31 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SNX27-PX (156-265)

Plasmid

#119106

Purpose

Bacterial expression of human phox homology (PX) domain, SNX27-PX (156-265)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX27-PX (156-265) (SNX27 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST53-Arl13b20

Plasmid

#40876

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted Arl N-terminal domain; added stop codon

Promoter

CMV

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only