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Plasmid#78550PurposeFor expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.DepositorTypeEmpty backboneUseRNAiTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only
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pKS011
Plasmid#65464PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherryDepositorInsertMBP-Ntag-mCherry
UseTagsFLAG and MBPExpressionBacterialMutationPromoterpl-TetOAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKS012
Plasmid#65465PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherryDepositorInsertNtag-mCherry
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterpl-TetOAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTE4938
Plasmid#107527PurposeExpresses Fn crRNA and mCherry in mammalian cells.DepositorInsertsFn crRNA
mCherry
UseTagsExpressionMammalianMutationPromoterCBh and human U6Available sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE4561
Plasmid#107525PurposeExpresses Lb crRNA and mCherry in mammalian cells.DepositorInsertsLb crRNA
mCherry
UseTagsExpressionMammalianMutationPromoterCBh and human U6Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE3179
Plasmid#107528PurposeExpresses Mb crRNA and mCherry in mammalian cells.DepositorInsertsMb crRNA
mCherry
UseTagsExpressionMammalianMutationPromoterCBh and human U6Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
LLP618_αGCN4-KRAB-CO
Plasmid#211782PurposeSunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertaGCN4-mCherry
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect mCherr…PromoterpEF1a and pSV40Available sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pRN11
Plasmid#84455Purposeshuttle plasmid for sarA P1-mCherry expressionDepositorInsertmCherry
UseShuttle vector e.coli-s.aureus, expression of sgf…TagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
Plasmid#198417PurposeExpresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeastDepositorInsertOsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBLO1806_Cas9_2xNLS_human
Plasmid#74493PurposeHuman codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNADepositorInsertCas9
UseTagst2a mCherryExpressionMammalianMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
p140Tol2-Olactb:SABER
Plasmid#226829PurposeExpression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.DepositorInsertsBFP-SABER
mCherry-t2A-CreERT2
UseZebrafish expressionTagsExpressionMutationPromoterOlactb and hsp70Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB5230
Plasmid#215496PurposeA pAde6 backbone integrating plasmid expressing Pil1-mCherry-SpAtg8(1-115) under the inducible promoter 41nmt1DepositorInsertPil1-mCherry-SpAtg8(1-115)
UseTagsExpressionYeastMutationPromoterP41nmt1Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
GA46
Plasmid#196614PurposePlasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeastDepositorInsertATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUGP1.1
Plasmid#196615PurposePlasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeastDepositorInsertUGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterNative promoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD066
Plasmid#84032PurposeVector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the elastase promoter and Venus under the crystalin promoter in zebrafishDepositorInsertsCre
Venus
UseCre/Lox; Zebrafish transgenesisTagsmCherryExpressionMutationPromoterCrystalin and ElastaseAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
FKBP_LD 0_TEV
Plasmid#58877PurposeMESA protease chain with Rapamycin FKBP ectodomain and no linker domainDepositorInsertMESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion
UseAAVTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-SS-338
Plasmid#68795PurposeSingle construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry markerDepositorInsertsC-Terminal Sensor 1 V1
mCherry
ZF9 Op x6
EGFP
N-Terminal Sensor 1 V2
UseTagsIRES, NLS, flag, and nlsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only