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Showing: 1221 - 1240 of 1683 results

  8. pCRISPRyl

    Plasmid
    #70007
    Purpose
    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al ACS Synth Biol. 2015 Dec 29.
    Inserts
    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70007-plasmid-map-sequence-id-137578

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  9. UCT1m

    Plasmid
    #121041
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UCT-part guide RNA (Stanley Qi sequence)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121041-plasmid-map-sequence-id-245418

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  11. M-tdTom-SP

    Plasmid
    #48677
    Purpose
    Mammalian tdTomato activation reporter for SP with GTCCCCTCCACCCCACAGTG protospacer
    Depositor
    George Church
    Article
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Insert
    TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, SP-PAM, and tdTomato reporter. Compatible with S. pyogenes Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Sp6
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pRH2502

    Plasmid
    #84379
    Purpose
    Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter
    Depositor
    Robert Husson
    Article
    Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw
    Insert
    dcas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Aspartate at position 10 changed to Alanine, Hist…
    Promoter
    uv15tetO
    Available since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    84379-plasmid-map-sequence-id-173256

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Showing: 1221 - 1240 of 1683 results