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Showing: 2481 - 2520 of 44046 results

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C514-E47: attB5r-Halotag-nostop-attB1r

Plasmid

#162963

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Halotag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLA CMV N-HA RALB WT

Plasmid

#50989

Purpose

Expresses RALB in mammalian cells

Depositor

Anna Sablina

Article

Simicek et al Nat Cell Biol. 2013 Oct;15(10):1220-30.

Insert

RALB (RALB Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
pCfB6684

Plasmid

#106154

Purpose

EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB6682

Plasmid

#106152

Purpose

EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2_(-3kb)nrg1_typeII:GFP-CAAX

Plasmid

#199394

Purpose

pDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region control

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR

Use

Tol2 gateway destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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GLN, Linear-3, PolyU(1)

Plasmid

#166984

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(1)

Expression

Mammalian

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r

Plasmid

#162958

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

triple FLAG epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB6679

Plasmid

#106158

Purpose

EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6638, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
C234-E08: attB2r-3xFLAG-attB3

Plasmid

#162912

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal triple-FLAG epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

triple FLAG epitope tag

Use

Synthetic Biology

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C514-E44: attB5r-SNAP-nostop-attB1r

Plasmid

#162962

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SNAP tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r

Plasmid

#162967

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Dual Strep2 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB6681

Plasmid

#106157

Purpose

EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT W63A

Plasmid

#90246

Purpose

Expresses YFP-HA-DAT W63A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated W63 to A

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT R60A

Plasmid

#90245

Purpose

Expresses YFP-HA-DAT R60A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated R60 to A

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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hopscotch

Plasmid

#37284

Depositor

Norbert Perrimon

Article

Binari et al Genes Dev. 1994 Feb 1;8(3):300-12.

Insert

hopscotch (hop Fly)

Expression

Bacterial

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pIL2R FAK Y397F

Plasmid

#50525

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

IL-2R

Expression

Mammalian

Mutation

Y397F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCS2+-tdmito-HyPerFLEX

Plasmid

#217567

Purpose

Mammalian expression HyPerFLEX targeted to the mitochondria

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

tdmito-HyPerFLEX

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC2+-HyPerFLEX-Lifeact

Plasmid

#217568

Purpose

Mammalian expression HyPerFLEX targeted to actin filaments

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX-Lifeact

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0009

Plasmid

#227572

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CYP716A392 (A357L)_35S

Expression

Plant

Mutation

A357L, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0010

Plasmid

#227573

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CYP716A392 (H424R)_35S

Expression

Plant

Mutation

H424R, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0018

Plasmid

#227574

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CYP716A392 (A285V)_35S

Expression

Plant

Mutation

A285V, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0012

Plasmid

#227575

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CYP716A393 (A285G)_35S

Expression

Plant

Mutation

A285G, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0014

Plasmid

#227576

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CYP716A393 (A357L)_35S

Expression

Plant

Mutation

A357L, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0015

Plasmid

#227577

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CYP716A393 (H424R)_35S

Expression

Plant

Mutation

H424R, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPDG1CB0021

Plasmid

#227578

Purpose

Level 1 - Position 2 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_ CYP716A393 (A285V)_35S

Expression

Plant

Mutation

A285V, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPHS1CB0029

Plasmid

#227556

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoMAS (I367M)_35S

Expression

Plant

Mutation

I367M, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPHS1CB0030

Plasmid

#227557

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoMAS (E371D)_35S

Expression

Plant

Mutation

E371D, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPHS1CB0031

Plasmid

#227558

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoMAS (I367M/E371D)_35S

Expression

Plant

Mutation

I367M / E371D, BsaI sites removed by silent mutat…

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPHS1CB0001

Plasmid

#227559

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoTXSS (G380T)_35S

Expression

Plant

Mutation

G380T, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPHS1CB0002

Plasmid

#227560

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoTXSS (D385E)_35S

Expression

Plant

Mutation

D385E, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPHS1CB0003

Plasmid

#227561

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoTXSS (H492Q)_35S

Expression

Plant

Mutation

H492Q, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPHS1CB0004

Plasmid

#227562

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoTXSS (P751A)_35S

Expression

Plant

Mutation

P751A, BsaI sites removed by silent mutation

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPHS1CB0017

Plasmid

#227563

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoTXSS (D385E/H492Q)_35S

Expression

Plant

Mutation

D385E / H492Q, BsaI sites removed by silent mutat…

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPHS1CB0020

Plasmid

#227564

Purpose

Level 1 - Position 1 with 35S promoter and terminator for plant expression, with mutations in CDS

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

35Sshort_TMV_CoTXSS (G380T/D385E/H492Q/P751A)_35S

Expression

Plant

Mutation

G380T / D385E / H492Q / P751A, BsaI sites removed…

Promoter

35Sshort_TMV (pICH51277)

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPMS0CM0026

Plasmid

#227519

Purpose

L0 part - CDS (without STOP codon)

Depositor

Nicola Patron

Article

Golubova et al Nat Commun. 2025 Jul 28;16(1):6941. doi:

Insert

CoMAS (Calendula officinalis mixed-amyrin synthase)

Expression

Plant

Mutation

BsaI sites removed by silent mutation

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-PM-deltaCKAR

Plasmid

#222429

Purpose

FRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Plasma membrane delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Nuc-deltaCKAR

Plasmid

#222430

Purpose

FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Nucleus delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Golgi-deltaCKAR

Plasmid

#222428

Purpose

FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Golgi delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p663-UBC-miniTurbo-V5-CSNK1G3_IDG-K

Plasmid

#214460

Purpose

Gateway destination clone of CSNK1G3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CSNK1G3 (CSNK1G3 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p663-UBC-miniTurbo-V5-CSNK1G1_IDG-K

Plasmid

#214458

Purpose

Gateway destination clone of CSNK1G1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CSNK1G1 (CSNK1G1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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