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We narrowed to 5,797 results for: plasmid dna

Showing: 3101 - 3150 of 5797 results

  15. pIK208

    Plasmid
    #65630
    Purpose
    sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"
    Depositor
    Iskra Katic
    Article
    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0
    Insert
    U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available Since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    65630-plasmid-map-sequence-id-111541

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  19. pIK137

    Plasmid
    #65632
    Purpose
    Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment
    Depositor
    Iskra Katic
    Article
    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0
    Insert
    Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available Since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    65632-plasmid-map-sequence-id-111543

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  20. pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

    Plasmid
    #69232
    Purpose
    Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
    Depositor
    Scot Wolfe
    Article
    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69232-plasmid-map-sequence-id-122311

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  25. pL0-EF-54730

    Plasmid
    #202163
    Purpose
    Level 0 plasmid containing a terminator sequence from gene 54730 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    54730 terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    None
    Promoter
    Available Since
    Sept. 6, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202163-plasmid-map-sequence-id-406454

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  26. pL0_AC-27877

    Plasmid
    #202110
    Purpose
    Level 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    27877 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter sequence mutated to remove sapI sites fo…
    Promoter
    Available Since
    Aug. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202110-plasmid-map-sequence-id-405522

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  27. pL0_AC-Fld

    Plasmid
    #202125
    Purpose
    Level 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    Fld promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter sequence mutated to remove bsaI site for…
    Promoter
    Available Since
    Aug. 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202125-plasmid-map-sequence-id-405541

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  28. pL0_AC-54730

    Plasmid
    #202109
    Purpose
    Level 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    54730 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter sequence mutated to remove bsaI site for…
    Promoter
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202109-plasmid-map-sequence-id-405433

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  29. pL0_AC-51183

    Plasmid
    #202108
    Purpose
    Level 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    51183 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter sequence mutated to remove sapI sites fo…
    Promoter
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202108-plasmid-map-sequence-id-405432

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  30. pL0_AC-GEF

    Plasmid
    #202107
    Purpose
    Level 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    GEF promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter sequence mutated to remove sapI site for…
    Promoter
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202107-plasmid-map-sequence-id-405430

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  31. pL0_AC-47740

    Plasmid
    #202106
    Purpose
    Level 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    47740 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter sequence mutated to remove bsaI site for…
    Promoter
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202106-plasmid-map-sequence-id-405429

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  32. pL0_AC-BCA

    Plasmid
    #202105
    Purpose
    Level 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    BCA promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter sequence mutated to remove sapI sites fo…
    Promoter
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202105-plasmid-map-sequence-id-405428

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  33. pL0_AC-epyC

    Plasmid
    #202104
    Purpose
    Level 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    epyC promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter sequence mutated to remove bsaI sites fo…
    Promoter
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202104-plasmid-map-sequence-id-405424

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  34. pSCL.040

    Plasmid
    #184974
    Purpose
    Test effect of extending a1/a2 on ADE2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2
    Use
    Tags
    Expression
    Yeast
    Mutation
    ADE2 donor P272stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184974-plasmid-map-sequence-id-376705

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  35. pSCL.109

    Plasmid
    #184978
    Purpose
    Test effect of extending a1/a2 on LYP1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 27 v1
    Use
    Tags
    Expression
    Yeast
    Mutation
    LYP1 donor E27stop, a1/a2 length extended to 27 bp
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184978-plasmid-map-sequence-id-376679

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  36. pSCL.107

    Plasmid
    #184976
    Purpose
    Test effect of extending a1/a2 on CAN1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 27 v1
    Use
    Tags
    Expression
    Yeast
    Mutation
    CAN1 donor G444stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184976-plasmid-map-sequence-id-376676

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  37. pSCL.111

    Plasmid
    #184980
    Purpose
    Test effect of extending a1/a2 on TRP2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1
    Use
    Tags
    Expression
    Yeast
    Mutation
    TRP2 donor E64stop, a1/a2 length extended to 27 bp
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184980-plasmid-map-sequence-id-376669

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  38. pSCL.113

    Plasmid
    #184982
    Purpose
    Test effect of extending a1/a2 on FAA1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
    Use
    Tags
    Expression
    Yeast
    Mutation
    FAA1 donor P233stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184982-plasmid-map-sequence-id-376532

    Enlarge

  39. pSCL.039

    Plasmid
    #184973
    Purpose
    Test effect of extending a1/a2 on ADE2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
    Use
    Tags
    Expression
    Yeast
    Mutation
    ADE2 donor P272stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184973-plasmid-map-sequence-id-376529

    Enlarge

  40. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid
    #69233
    Purpose
    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
    Depositor
    Scot Wolfe
    Article
    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…
    Expression
    Mammalian
    Mutation
    Arginine 1335 is changed to Lysine
    Promoter
    Available Since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    69233-plasmid-map-sequence-id-122312

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Showing: 3101 - 3150 of 5797 results