We narrowed to 15,366 results for: LEA

1. rAAV-U6-MeCP2shRNA-CamKIIa-mCherry

   Plasmid

   #169704

   Purpose

   MeCP2 Knockdown

   Depositor

   Ana Oliveira

   Article

   Gulmez Karaca et al Neurobiol Learn Mem. 2018 Mar;149:84-97.

   Insert

   shRNA against MeCp2 (Mecp2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTXB1-EXO1b D173A

   Plasmid

   #68269

   Purpose

   Bacterial expression of human EXO1 D173A (catalytically-dead) mutant

   Depositor

   Stefano Ferrari

   Article

   Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10

   Insert

   EXO1 (EXO1 Human)
   

   Use

   Tags

   Mxe intein/chitin binding domain

   Expression

   Bacterial

   Mutation

   catalytically-dead D173A. Please see depositor co…

   Promoter

   T7

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pNUT N6His K206E/K534A hTFNG

   Plasmid

   #70133

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe

   Depositor

   Anne Mason, Ross MacGillivray

   Article

   Halbrooks et al Biochemistry. 2003 Apr 8;42(13):3701-7.

   Insert

   N6His K206e/K534A hTFNG (TF Human)
   

   Use

   Tags

   N-terminal signal peptide, 4 aa link, 6 His, Fact…

   Expression

   Mammalian

   Mutation

   changed Lys206 to a glutamic acid and Lys534 to a…

   Promoter

   SV40

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-dSpCas9-HF1-VP64-6xHis

   Plasmid

   #92118

   Purpose

   Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   T7

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-Flag-SpCas9-HF1 (without sgRNA)

   Plasmid

   #92102

   Purpose

   Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT-ubb-NLS-BirA-2a-mCherry

   Plasmid

   #79886

   Purpose

   Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-Drosha270-1374delNLS2

   Plasmid

   #62532

   Purpose

   N-terminal aa1-269 and nuclear localization signal 2 of Drosha are deleted

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   truncated human Drosha mutant (DROSHA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-269 and nuclear localizatio…

   Promoter

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

8. hFlt1 Y1333F

   Plasmid

   #86037

   Purpose

   human VEGFR1 with Y to F mutation at position 1333

   Depositor

   Christie Thomas

   Article

   Raikwar et al PLoS One. 2014 Nov 11;9(11):e112794. doi

   Insert

   human Flt1 Y1333F (FLT1 Human)
   

   Use

   Tags

   FLAG, HA, and Myc

   Expression

   Mammalian

   Mutation

   Y1333F; HA tag is inserted downstream of signal …

   Promoter

   CMV

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. hFLT1 delta 754-763

   Plasmid

   #86035

   Purpose

   human VEGFR1 with a 10 amino acid deletion in EC close to TM domain

   Depositor

   Christie Thomas

   Article

   Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

   Insert

   human Flt1 delta754-763 aa (FLT1 Human)
   

   Use

   Tags

   FLAG, HA, and Myc

   Expression

   Mammalian

   Mutation

   delta 754-763

   Promoter

   CMV

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2+-FLAG3-drN-hsAgo2

    Plasmid

    #221724

    Purpose

    Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for zebrafish Ago2 N-domain.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drN-hsAgo2 (AGO2 Human)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    Swap of N-domain for zebrafish Ago2 N-domain

    Promoter

    simian CMV IE94

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+-FLAG3-hsN-drAgo2

    Plasmid

    #221717

    Purpose

    Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsN-drAgo2 (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    Swap of N-domain for human Ago2 N-domain

    Promoter

    simian CMV IE94

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+-FLAG3-hsAgo2EF-DY

    Plasmid

    #221723

    Purpose

    Expression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsAgo2EF-DY (AGO2 Human)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    E637D and F666Y in active site

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pS97splitRBP_S2-Enz

    Plasmid

    #183768

    Purpose

    Expresses SHIP2-Enzyme domain flanked by RBP

    Depositor

    William Kerr

    Article

    Sudan et al Front Immunol. 2022 May 4;13:830961. doi

    Insert

    SHIP2 (INPPL1 Human)
    

    Use

    Tags

    6HIS, RBP (C-terminal) HRV3C cleavage site, and R…

    Expression

    Bacterial

    Mutation

    includes aa K414 to aa V872 only

    Promoter

    T7lac

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xEGFP-mMeikin(1-391, E156R, R159E) - hRad21(142-275) - mMeikin(387-434)

    Plasmid

    #174712

    Purpose

    Mammalian expression of mouse Meikin with wild-type Separase cleavage site eliminated and C-terminal Separase cleavage site inserted

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin (Meikin Mouse)
    

    Use

    Tags

    3xEGFP

    Expression

    Mammalian

    Mutation

    mMeikin: 1-391, E156R, R159E; hRad21: 142-275; mM…

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-HA-NONO

    Plasmid

    #127655

    Purpose

    Over-express HA-tagged human NONO

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

    Insert

    NONO (NONO Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINE GFP nanobody:Halo:His6

    Plasmid

    #111090

    Purpose

    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

    Depositor

    Lennart Wirthmueller

    Article

    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

    Insert

    GFP Nanobody
    

    Use

    Tags

    Halo and His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7 promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-BSD-tet-mEGFP-MIB1

    Plasmid

    #140241

    Purpose

    Lentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline treatment

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    Tet-inducible mEGFP-MIB1 (MIB1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-AKAP95-FRB

    Plasmid

    #138254

    Purpose

    Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.

    Depositor

    Jin Zhang

    Article

    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

    Insert

    AKAP95-FRB (AKAP8 Human)
    

    Use

    Tags

    FRB rapamycin binding domain and HA epitope

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. NLS-4LNDD-pcDNA3.1(+)

    Plasmid

    #172313

    Purpose

    Expresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cells

    Depositor

    Joshua Rosenthal

    Article

    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

    Insert

    Lambda N peptide - human ADAR2 Deaminase Domain
    

    Use

    Tags

    6xHis, Flag, and Nuclear Localization Signal of S…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. AF20-double-tag

    Plasmid

    #108389

    Purpose

    Express human PC1

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    eGFP-TEV cleavage-S15 and mcherry-TEV cleavage-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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