Addgene

We narrowed to 7,339 results for: Lif

Showing: 1241 - 1260 of 7339 results

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pDEST_6xUAS:miRFP670-cetn4

Plasmid

#192357

Purpose

zfCETN4 tagged with miRFP670, expressed under the UAS promoter

Depositor

Darren Gilmour

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

CETN4 (cetn4 Zebrafish)

Use

Tags

miRFP670

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-RT001-LVB

Plasmid

#163381

Purpose

Second generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

LaG17-VEEGFRtm-BFP (LVB)

Use

Lentiviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJCC_039 FnCas12a

Plasmid

#171666

Purpose

For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

FnCas12a

Use

Tags

6X His and sortase A sorting motif

Expression

Bacterial

Mutation

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR R4 ATCC LacZ GCTT

Plasmid

#173668

Purpose

Destination vector for Golden Gate Cloning assemblies, blue-white screening

Depositor

Eric Marois

Article

Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

Type

Empty backbone

Use

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-RT002-LV

Plasmid

#163382

Purpose

2nd generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

LaG17-VEGFRtm (LV)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g5

Plasmid

#80788

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-hChR2(H134R)-EYFP

Plasmid

#114367

Purpose

expresses channelrhodopsin 2 fused with EYFP

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

hChR2(H134R)

Use

Tags

EYFP

Expression

Mammalian

Mutation

the Histidine at amino acid position 134 was chan…

Promoter

pCAG

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g6

Plasmid

#80789

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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OSCA1.8

Plasmid

#136594

Purpose

Expresses the mechanosensitive channel OSCA1.8 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

OSCA1.8 (AT1G32090 Mustard Weed)

Use

Tags

pIRES2-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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15x-QUAS-dTomato-T2A-GCaMP6s

Plasmid

#130666

Purpose

QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

Depositor

Leslie Vosshall

Article

Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

Insert

15xQUAS-dTomato-T2A-GCaMP6s

Use

Ae. aegypti expression

Tags

Expression

Insect

Mutation

Promoter

15xQUAS-TATA

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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MCP-ADAR2-DD

Plasmid

#170124

Purpose

Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MCP-ADAR2-DD-NES

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a core promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-RT006-GCN4-HaloPM

Plasmid

#163386

Purpose

To express 4X sGCN4 module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

4XGCN4-Halotag-PDGFRtm-2A-mCherry (GCN4-HaloPM)

Use

Lentiviral

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMS84 (U6p::GGACAGTCCTGCCGAGGTGG)

Plasmid

#193852

Purpose

Encodes the guide RNA targeting the sequence GGACAGTCCTGCCGAGGTGG

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

Guide RNA

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEF HaloTag-SYB2

Plasmid

#197282

Purpose

Encodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYB2 and HaloTag (Vamp2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Q76V and F77W

Promoter

EF1alpha

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-GCaMP6s

Plasmid

#166606

Purpose

Encodes Cre-dependent GCaMP6s under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

GCaMP6s

Use

AAV

Tags

Expression

Mutation

Promoter

tetracycline response element

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_PACLight1

Plasmid

#197864

Purpose

Mammalian expression of PACLight1 biosensor for PACAP (Pituitary Adenylate Cyclase Activating Peptide)

Depositor

Tommaso Patriarchi

Article

Cola et al Elife. 2024 Aug 15;13:RP96496. doi: 10.7

Insert

PACLight1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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nontarget S. pyogenes scaffold CARGO array

Plasmid

#191319

Purpose

Contains an array of nontargeting guide RNAs

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPe-SH1446

Plasmid

#112507

Purpose

Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

Depositor

Simon Newstead

Article

Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

Insert

POT family transporter

Use

Tags

C terminal TEV GFP octa-His affinity tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry_SNAP_SNAPV_PACT_Active

Plasmid

#173072

Purpose

Active SNAP tag at the centrosome

Depositor

Heidi Hehnly

Article

Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

Insert

mCherry-SNAP-SNAP-Varied-PACT

Use

Tags

mCherry-SNAP-SNAP-Varied

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Flp-DOG

Plasmid

#80084

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

Flp-DOG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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