We narrowed to 10,941 results for: NSI;

1. pYDS2.0_AT118-L

   Plasmid

   #222847

   Purpose

   Yeast-display plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-TetOneZeo-ORF18(WT)-3xFLAG

   Plasmid

   #129746

   Purpose

   Dox inducible expression of KSHV ORF18-3xFLAG in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

   Insert

   ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3Gs and Tet inducible promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Yellow Cameleon-Nano140/pcDNA3

   Plasmid

   #51966

   Purpose

   mammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano140
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b_EcSSB_1

   Plasmid

   #78203

   Purpose

   Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

   Depositor

   Martin Webb

   Article

   Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

   Insert

   ssb (G26C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G26C (please see depositor comment below)

   Promoter

   T7

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUWR-CadTS-C

   Plasmid

   #58326

   Purpose

   Destination vector for inserting ubi-CadTS control to Drosophila melanogaster genome

   Depositor

   Denise Montell

   Article

   Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

   Insert

   E-cadherin tension sensor control
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   poly-ubiquitin

   Available since

   Aug. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

6. Yellow Cameleon-Nano50/pcDNA3

   Plasmid

   #51964

   Purpose

   mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

   Depositor

   Takeharu Nagai

   Article

   Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

   Insert

   Yellow Cameleon-Nano50
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTSSX-STC

   Plasmid

   #115668

   Purpose

   E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag

   Depositor

   David Kramer

   Article

   Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459

   Insert

   small tetraheme cytochrome c
   

   Use

   Tags

   Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGCNK58

   Plasmid

   #202461

   Purpose

   Plasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cells

   Depositor

   Arthur Palmer, Stephen Harrison

   Article

   Weiss et al Nature. 1990 Oct 11;347(6293):575-8. doi

   Insert

   Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-PI(WT)-Src(YF)-mCherry

   Plasmid

   #87357

   Purpose

   PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   Src isoform 2 (Src Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Y535F

   Promoter

   CMV

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUE1-TSP-ORF68 R443A

    Plasmid

    #162652

    Purpose

    Expresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acid R443 mutated to alanine

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe-PI(WT)-Src(KD)-mCherry

    Plasmid

    #87358

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src (Src Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    D388R

    Promoter

    CMV

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. RS18960_pMQ30_KO_Kan

    Plasmid

    #185393

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS18960 upstream flanking homology region
    RR42_RS18960 downstream flanking homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RS17060_pMQ30_KO_Kan

    Plasmid

    #185396

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS17060 upstream flanking homology region
    RR42_RS17060 downstream flanking homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RS17055_pMQ30_KO_Kan

    Plasmid

    #185398

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS17055 upstream flanking homology region
    RR42_RS17055 downstream flanking homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. K8.1Pr-pGL4.16

    Plasmid

    #131037

    Purpose

    Firefly Luciferase reporter for K8.1 promoter from KSHV

    Depositor

    Britt Glaunsinger

    Article

    Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

    Insert

    KSHV K8.1 100 bp promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF-XMAS-2xStop

    Plasmid

    #164412

    Purpose

    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGST-TEV-yVms1(1-417,delta(38-69))

    Plasmid

    #128739

    Purpose

    Bacterial overexpression and crystallization

    Depositor

    Chris Hill

    Article

    Nielson et al Mol Cell. 2017 Nov 16;68(4):673-685.e6.

    Insert

    Vms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    residues 1-417 with deletion of 38-69

    Promoter

    T7

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. HpMscS

    Plasmid

    #78310

    Purpose

    H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag

    Depositor

    Douglas Rees

    Article

    Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:

    Insert

    Mechanosensitive channel of small conductance
    

    Use

    Tags

    6x His Tag and FLAG Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2

    Plasmid

    #91873

    Purpose

    Photo-activatable/light sensitive Vav2

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    VAV2 DH/PH/ZF (Vav2 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUWR-CadTS

    Plasmid

    #58325

    Purpose

    Destination vector for inserting ubi-CadTS to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    E-cadherin tension sensor
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    poly-ubiquitin

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only