We narrowed to 2,847 results for: PHI-1

1. U6_sgRNA_CAG_hSt1Cas9_LMG18311

   Plasmid

   #136653

   Purpose

   A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD9:LMG18311 chimera
   sgRNA for St1Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and hU6

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-EGFP-DmeIF4E

   Plasmid

   #79258

   Purpose

   Expresses EGFP-tagged DmeIF4E in S2 cells

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

   Insert

   eIF4E (eIF4E1 Fly)
   

   Use

   Schneider 2 cell expression

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Ac5 promoter

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPQD1CB0785

   Plasmid

   #177070

   Purpose

   MoClo Level 1, position 2, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1-mEGFP-CCR4 isoform A (HK52)

   Plasmid

   #206439

   Purpose

   CCR4 expression in Schneider cells for imaging

   Depositor

   Mandy Jeske

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   CCR4 (twin Fly)
   

   Use

   Tags

   mEGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1-PH-mCherry-CAF1 isoform A (HK96)

   Plasmid

   #206454

   Purpose

   CAF1 expression in Schneider cells for imaging

   Depositor

   Mandy Jeske

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   CAF1 (Pop2 Fly)
   

   Use

   Tags

   PH mcherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAc5.1-OST4-mCherry-CAF1 isoform A (HK184)

   Plasmid

   #206476

   Purpose

   CAF1 expression in Schneider cells for imaging

   Depositor

   Mandy Jeske

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   CAF1 (Pop2 Fly)
   

   Use

   Tags

   OST4 mcherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_ABE‐NG‐loop

   Plasmid

   #187057

   Purpose

   ABEmax-SpCas9-NG-loop

   Depositor

   Renzhi Han

   Article

   Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

   Insert

   ABE‐NG‐loop
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pF3BGX-T2A-p65-AD

   Plasmid

   #138395

   Purpose

   RMCE vector for generating C-terminal T2A-p65-AD fusion (split GAL4)

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Insert

   T2A-p65-AD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDB302

   Plasmid

   #113673

   Purpose

   expresses Cth SUMO protease in Escherichia coli

   Depositor

   Christopher Bahl

   Article

   Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

   Insert

   Cth SUMO protease
   

   Use

   Tags

   10-His

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-934

   Promoter

   T7

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti‐puro‐OgRNA_mdxE53

    Plasmid

    #187054

    Purpose

    SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CS2 luc Utrn

    Plasmid

    #13500

    Depositor

    Stephen Tapscott

    Article

    Rosenberg et al J Cell Biol. 2006 Oct 9. 175(1):77-85.

    Insert

    Utrophin (Utrn Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Contains 5 copies of nt 10657-10693 of NM_011682.

    Promoter

    Available since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

12. GST-CLCb

    Plasmid

    #47404

    Depositor

    Peter McPherson

    Article

    Legendre-Guillemin et al J Biol Chem. 2005 Feb 18;280(7):6101-8.

    Insert

    CLCb
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pAc5.1C-Fluc-nerfin_E

    Plasmid

    #146190

    Purpose

    Insect Expression of nerfin-3UTR

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

    Insert

    nerfin-3UTR (nerfin-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFd029.R2.RFP

    Plasmid

    #137972

    Purpose

    Expresses Prochlorococcus marinus NATL1A Fd 1 fused to mRFP

    Depositor

    Jonathan Silberg

    Article

    Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

    Insert

    Prochlorococcus marinus NATL1A ferredoxin 1 fused to monomeric RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFd030.R2.RFP

    Plasmid

    #137973

    Purpose

    Expresses Prochlorococcus marinus NATL2A Fd 1 fused to mRFP

    Depositor

    Jonathan Silberg

    Article

    Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

    Insert

    Prochlorococcus marinus NATL2A ferredoxin 1 fused to monomeric RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFd031.RFP

    Plasmid

    #137974

    Purpose

    Expresses Prochlorococcus marinus MIT9211 Fd 1 fused to mRFP

    Depositor

    Jonathan Silberg

    Article

    Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

    Insert

    Prochlorococcus marinus MIT9211 ferredoxin 1 fused to monomeric RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KHBD00370

    Plasmid

    #34485

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8409 (Su(var)205 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pT22gCfpUbiG4FER4P2A(#249)

    Plasmid

    #184070

    Purpose

    cyclofen-inducible GAL4 activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

    Insert

    Gal4bdFF-ERT2-P2A
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-puro_GCaMP6s

    Plasmid

    #164589

    Purpose

    Stable integration and expression of GCaMP6s in mammalian cells

    Depositor

    Joseph Hyser

    Article

    Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

    Insert

    GCaMP6s
    

    Use

    Lentiviral

    Tags

    6xHis, T7 tag, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpyTag-β-Lactamase-SpyCatcher

    Plasmid

    #52656

    Purpose

    Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.

    Depositor

    Mark Howarth

    Article

    Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2

    Insert

    TEM-1 β-Lactamase
    

    Use

    Tags

    SpyCatcher and SpyTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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