Addgene

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pAct-Cas9

Plasmid

#62209

Purpose

Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

cas9

Use

Tags

Expression

Insect

Mutation

Promoter

act5C

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T1

Plasmid

#41819

Purpose

Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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ELK1 pcDNA3.1/HisC

Plasmid

#121093

Purpose

Expression of full length ELK1 in mammalian cells

Depositor

Paul Herring

Article

Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

Insert

ELK1 (Elk1 Mouse)

Use

Tags

6XHis-Omni-xpress

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTJV1ScKm-rpoB

Plasmid

#166980

Purpose

Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Insert

NeoR/KanR

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAJE-Ma pylRS nitroY/haloY-F5

Plasmid

#225684

Purpose

Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.

Depositor

Ryan Mehl

Inserts

M. alvus PylRS 3-NY/HaloY F5
M. alvus Pyl-tRNA(6)

Use

Methanomethylophilus alvus

Tags

Expression

Mutation

CTG125CTT, N166A, V168S, A223C, W239R

Promoter

GlnS and Lpp

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pBTK622

Plasmid

#180592

Purpose

tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

Depositor

Jeffrey Barrick

Article

Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

Inserts

tdk
kanR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin WT

Plasmid

#45969

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin (Prkn Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression

Promoter

tac

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T2

Plasmid

#41820

Purpose

Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pLBH545_Tet-Cas14a1_Locus

Plasmid

#112501

Purpose

Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pUB_FLuc100---MS2

Plasmid

#210574

Purpose

Expresses Optimal Firefly with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal Firefly luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Human p150,95

Plasmid

#8633

Depositor

Timothy Springer

Article

Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

Insert

Human p150,95 (ITGAX Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pLBH559_Tet-HisCas14a1Locus

Plasmid

#112502

Purpose

Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide B

Plasmid

#194011

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide B)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide A

Plasmid

#194008

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide A)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide B

Plasmid

#194009

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide B)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide A

Plasmid

#194010

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide A)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET51b-NAD-cpLuc2

Plasmid

#135629

Purpose

Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)

Depositor

Kai Johnsson

Article

Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

Insert

NAD-cpLuc2 sensor

Use

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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Integrin-β6 Headpiece I270C

Plasmid

#159638

Purpose

Contains integrin beta 6 residues 1–474 with I270C mutation

Depositor

Timothy Springer

Article

Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

Insert

Integrin beta 6 headpiece I270C

Use

Tags

6x His

Expression

Mammalian

Mutation

I270C

Promoter

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Integrin-αv Headpiece M400GC

Plasmid

#159637

Purpose

Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

Depositor

Timothy Springer

Article

Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

Insert

alpha v headpiece M400GC

Use

Tags

6x His and Strep II

Expression

Mammalian

Mutation

M400C

Promoter

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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mUAVbla:tetR 5′

Plasmid

#149379

Purpose

mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs

Depositor

Mark Pallen

Article

Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

Insert

tetA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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