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We narrowed to 1,524 results for: piggyBac

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Showing: 1241 - 1260 of 1524 results

  1. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197027
    Purpose
    Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized enRhCas12f1
    Expression
    Mammalian
    Mutation
    L270R
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197027-plasmid-map-sequence-id-388964

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  2. PB-TAC-ERP2-(N-HA)KRAS_G12D

    Plasmid
    #201254
    Purpose
    Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells
    Depositor
    Alexander Kleger
    Publication
    Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAS (KRAS Human)
    Use
    Piggybac transposon
    Tags
    HA-Tag
    Expression
    Mammalian
    Mutation
    changed Gly (G) 12 to Asp (D)
    Promoter
    TRE
    Available since
    Sept. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    201254-plasmid-map-sequence-id-408509

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  3. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

    Plasmid
    #134923
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134923-plasmid-map-sequence-id-267593

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  6. vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro)

    Plasmid
    #160044
    Purpose
    For expressing iaaH to convert auxin precursors (IAM or NAM) to auxin (IAA or NAA)
    Depositor
    Michael Elowitz
    Publication
    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iaaH (iaaH )
    Use
    Synthetic Biology
    Tags
    3xNLS-Citrine
    Expression
    Mammalian
    Promoter
    pEF1s
    Available since
    April 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    160044-plasmid-map-sequence-id-318469

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  8. TK4_SH4-1_targeting

    Plasmid
    #254452
    Purpose
    TK4 targeting vector containing left and right homology arms for the SH4-1 locus
    Depositor
    Marius Wernig
    Publication
    Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2
    Inserts
    mycNLS-mScarlet-T2A-mNeonGreen-PEST
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Piggybac
    Tags
    PEST, T2A-mycNLS-mTagBFP2, and c-myc-NLS
    Expression
    Mammalian
    Promoter
    CAG and EF1a
    Available since
    April 24, 2026
    Availability
    Academic Institutions and Nonprofits only
    254452-plasmid-map-sequence-id-507707

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  12. TK4_SH4-2_targeting

    Plasmid
    #254453
    Purpose
    TK4 targeting vector containing left and right homology arms for the SH4-2 locus
    Depositor
    Marius Wernig
    Publication
    Uenaka et al Cell Stem Cell. 2026 Feb 13:S1934-5909(2
    Inserts
    mycNLS-mScarlet-T2A-mNeonGreen-PEST
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Piggybac
    Tags
    PEST, T2A-mycNLS-mTagBFP2, and c-myc-NLS
    Expression
    Mammalian
    Promoter
    CAG and EF1a
    Available since
    April 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    254453-plasmid-map-sequence-id-505729

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  15. pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid
    #84257
    Purpose
    Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sp dCas9-ABI
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    84257-plasmid-map-sequence-id-160891

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  20. pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast

    Plasmid
    #205540
    Purpose
    Express HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance marker
    Depositor
    Michael Elowitz
    Publication
    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gag-MCP
    Use
    Piggybac
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    TRE3GS
    Available since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    205540-plasmid-map-sequence-id-408285

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Showing: 1241 - 1260 of 1524 results